Meiosis in Coprinus: characterization and activities of two forms of DNA polymerase during meiotic stages.

K Sakaguchi; B C Lu

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Meiosis in Coprinus: characterization and activities of two forms of DNA polymerase during meiotic stages.

机译：鸡腿菇减数分裂：减数分裂阶段两种形式的DNA聚合酶的表征和活性。

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Two forms of DNA polymerase have been studied in the basidiomycete Coprinus. DNA polymerase from basidiocarp tissues at zygotene-pachytene stage has been purified 3,500-fold and defined as DNA polymerase b by virtue of its insensitivity to N-ethylmaleimide and by its low molecular weight (76,000). This enzyme has optimal activity at pH 7.0 to 7.5, at 200 mM KCl, and at 25 degrees C incubation temperature. It can use polycytidylic acid-oligo(dG)12-18 as template primer in addition to homodeoxypolymers. The DNA polymerase a is mainly produced in the exponentially growing mycelium. It is sensitive to N-ethylmaleimide and has a temperature optimum at 35 degrees C. At the premeiotic S phase, activities from both polymerase a and polymerase b are found in cell-free extracts. The b enzyme is the only DNA polymerase produced during meiotic prophase. Its assayable activity exhibits two peaks, one at premeiotic S stage and one at pachytene. It is possible that DNA polymerase b is responsible for pachytene repairs involved in recombination.

机译：在担子菌属鸡腿菇中研究了两种形式的DNA聚合酶。由于对N-乙基马来酰亚胺不敏感并且分子量低（76,000），因此从合子-粗线期的果皮组织中提取的DNA聚合酶被纯化了3,500倍，被定义为DNA聚合酶b。该酶在pH 7.0至7.5、200 mM KCl和25摄氏度的孵育温度下具有最佳活性。除均聚脱氧聚合物外，它还可以使用聚胞苷酸-寡聚（dG）12-18作为模板底漆。 DNA聚合酶α主要在指数增长的菌丝体中产生。它对N-乙基马来酰亚胺很敏感，最适温度为35摄氏度。在减数分裂前S期，无细胞提取物中发现了聚合酶a和聚合酶b的活性。 b酶是减数分裂前期产生的唯一DNA聚合酶。它的可测定活性表现出两个峰，一个在减数分裂前期，一个在粗线期。 DNA聚合酶b可能负责重组中的粗线修复。

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《Molecular and Cellular Biology》 |1982年第7期|共6页
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K Sakaguchi; B C Lu;
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1. DNA polymerase mu interacts with a meiosis-specific RecA homolog Lim15 during meiosis in Coprinus cinereus. [J] . Yanai T, Sakamoto A, Iwabata Biochemical and Biophysical Research Communications . 2009,第1期

机译：DNA聚合酶μ在灰粉鬼伞的减数分裂过程中与减数分裂特异的RecA同源物Lim15相互作用。
2. Two X family DNA polymerases, lambda and mu, in meiotic tissues of the basidiomycete, Coprinus cinereus [J] . Sakamoto A, Iwabata K, Koshiyama A, Chromosoma . 2007,第6期

机译：担子菌，灰粉鬼伞的减数分裂组织中的两个X家族DNA聚合酶lambda和mu
3. Two X family DNA polymerases, λ and μ, in meiotic tissues of the basidiomycete, Coprinus cinereus [J] . Aiko Sakamoto, Kazuki Iwabata, Akiyo Koshiyama, Chromosoma . 2007,第6期

机译：担子菌，灰粉鬼伞的减数分裂组织中的两个X家族DNA聚合酶λ和μ
4. Cell-free incorporation of photo-crosslinkable p-benzoyl-L-phenylalanine into the polymerase subunits α and ε of Escherichia coli DNA polymerase III [C] . Kiyoshi Ozawa, Gottfried Otting, Nicholas E. Dixon Incorporating of the Australian Society for Biochemistry and Molecular Biology Combio . 2009

机译：无细胞掺入光交联的p-苯甲酰基-1-苯丙氨酸进入聚合酶亚基α和ε的大肠杆菌DNA聚合酶III
5. I. Comparison of translesion bypass of guanine-N2 monoadducts of mitomycin C and guanine -N7 monoadducts of 2,7-diaminomitosene by T7 exo-, Klenow exo-, eta and Klenow exo+ DNA polymerases. II. Structure-based design, synthesis, structure-conformation and structure -activity relationships studies of D-Phe-Pro-D-Arg-P1'-CONH2 tetrapeptides with inhibitory activity for thrombin. [D] . Clement, Cristina C. 2006

机译：I.通过T7 exo-，Klenow exo-，eta和Klenow exo + DNA聚合酶比较丝裂霉素C的鸟嘌呤-N2单加合物和2,7-二氨基光油的鸟嘌呤-N7单加合物的跨病变旁路。二。具有凝血酶抑制活性的D-Phe-Pro-D-Arg-P1'-CONH2四肽的基于结构的设计，合成，结构构象和结构-活性关系研究。
6. Meiosis in Coprinus: characterization and activities of two forms of DNA polymerase during meiotic stages. [O] . K Sakaguchi, B C Lu 1982

机译：鸡腿菇减数分裂：减数分裂阶段两种形式的DNA聚合酶的特征和活性。
7. Meiosis in Coprinus: characterization and activities of two forms of DNA polymerase during meiotic stages. [O] . Sakaguchi, K, Lu, B C 1982

机译：鸡腿菇减数分裂：减数分裂阶段两种形式的DNA聚合酶的特征和活性。

Meiosis in Coprinus: characterization and activities of two forms of DNA polymerase during meiotic stages.

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