Cloning and characterization of the low-affinity cyclic AMP phosphodiesterase gene of Saccharomyces cerevisiae.

J Nikawa; P Sass; M Wigler

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Cloning and characterization of the low-affinity cyclic AMP phosphodiesterase gene of Saccharomyces cerevisiae.

机译：酿酒酵母的低亲和力环状AMP磷酸二酯酶基因的克隆和鉴定。

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Saccharomyces cerevisiae contains two genes which encode cyclic AMP (cAMP) phosphodiesterase. We previously isolated and characterized PDE2, which encodes a high-affinity cAMP phosphodiesterase. We have now isolated the PDE1 gene of S. cerevisiae, which encodes a low-affinity cAMP phosphodiesterase. These two genes represent highly divergent branches in the evolution of phosphodiesterases. High-copy-number plasmids containing either PDE1 or PDE2 can reverse the growth arrest defects of yeast cells carrying the RAS2(Val-19) mutation. PDE1 and PDE2 appear to account for the aggregate cAMP phosphodiesterase activity of S. cerevisiae. Disruption of both PDE genes results in a phenotype which resembles that induced by the RAS2(Val-19) mutation. pde1- pde2- ras1- ras2- cells are viable.

机译：酿酒酵母包含两个编码环状AMP（cAMP）磷酸二酯酶的基因。我们先前分离并鉴定了PDE2，它编码高亲和力的cAMP磷酸二酯酶。现在，我们已经分离出酿酒酵母的PDE1基因，该基因编码低亲和力的cAMP磷酸二酯酶。这两个基因代表磷酸二酯酶进化中的高度分歧分支。包含PDE1或PDE2的高拷贝数质粒可以逆转带有RAS2（Val-19）突变的酵母细胞的生长停滞缺陷。 PDE1和PDE2似乎解释了酿酒酵母的总cAMP磷酸二酯酶活性。两种PDE基因的破坏都会产生类似于RAS2（Val-19）突变诱导的表型。 pde1- pde2- ras1- ras2-细胞是可行的。

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《Molecular and Cellular Biology》 |1987年第10期|共8页
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J Nikawa; P Sass; M Wigler;
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中图分类细胞生物学;
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1. Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae. [J] . T Toda, S Cameron, P Sass, Molecular and Cellular Biology . 1987,第4期

机译：BCY1的克隆与表征，一种在酿酒酵母中编码循环AMP依赖性蛋白激酶的调节亚基的基因座。
2. Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae. [J] . T Toda, S Cameron, P Sass, Molecular and Cellular Biology . 1987,第4期

机译：BCY1的克隆与表征，一种在酿酒酵母中编码循环AMP依赖性蛋白激酶的调节亚基的基因座。
3. Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae. [J] . T Toda, S Cameron, P Sass, Molecular and Cellular Biology . 1987,第4期

机译：BCY1的克隆与表征，一种在酿酒酵母中编码循环AMP依赖性蛋白激酶的调节亚基的基因座。
4. Molecular Cloning and Characterization of Photosystem II gene (BcppsbO) from Brassica Campestris ssp . Pekinensis [C] . LI Zhu-gang, WANG Guang-jin, ZHAO Ling-xia, International Agro-Biotechnology Conference; 2005; Heilongjiang(CN) . 2005

机译：甘蓝型油菜光系统II基因（BcppsbO）的分子克隆与鉴定。北京
5. Cloning and genetic characterization of two calcium-dependent membrane binding proteins in Saccharomyces cerevisiae. [D] . Kambouris, Nicholas George. 1991

机译：酿酒酵母中两个钙依赖性膜结合蛋白的克隆和遗传特征。
6. Cloning and characterization of the low-affinity cyclic AMP phosphodiesterase gene of Saccharomyces cerevisiae. [O] . J Nikawa, P Sass, M Wigler 1987

机译：酿酒酵母的低亲和力环状AMP磷酸二酯酶基因的克隆和鉴定。
7. Cloning and characterization of the low-affinity cyclic AMP phosphodiesterase gene of Saccharomyces cerevisiae. [O] . Nikawa, J, Sass, P, Wigler, M 1987

机译：酿酒酵母的低亲和力环状AMP磷酸二酯酶基因的克隆和鉴定。
8. Cloning and Characterization of Genes Encoding Proteins Involved in the TerminalStage of Vesicular Traffic in the Yeast 'Saccharomyces cerevisiae' [R] . Aalto, M. 1995

机译：酵母'酿酒酵母'中水泡交通终末期蛋白质编码基因的克隆与鉴定

Cloning and characterization of the low-affinity cyclic AMP phosphodiesterase gene of Saccharomyces cerevisiae.

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