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首页> 外文期刊>Molecular and Cellular Biology >A yeast mutation that stabilizes a plasmid bearing a mutated ARS1 element.
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A yeast mutation that stabilizes a plasmid bearing a mutated ARS1 element.

机译：一种酵母突变，可稳定带有突变ARS1元件的质粒。

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To identify the trans-acting factors involved in autonomously replicating sequence (ARS) function, we initiated a screen for Saccharomyces cerevisiae mutants capable of stabilizing a plasmid that contains a defective ARS element. The amm (altered minichromosome maintenance) mutations recovered in this screen defined at least four complementation groups. amm1, a mutation that has been studied in detail, gave rise to a 17-fold stabilization of one defective ARS1 plasmid over the level seen in wild-type cells. The mutation also affected the stability of at least one plasmid bearing a wild-type ARS element. amm1 is an allele of the previously identified TUP1 gene and exhibited the same pleiotropic phenotypes as other tup1 mutants. Plasmid maintenance was also affected in strains bearing a TUP1 gene disruption. Like the amm1 mutant, the tup1 disruption mutant exhibited ARS-specific plasmid stabilization; however, the ARS specificities of these two mutants differed. The recovery of second-site mutations that suppressed many of the tup1 phenotypes but not the increased plasmid maintenance demonstrates that the plasmid stability phenotype of tup1 mutants is not a consequence of the other defects caused by tup1.
机译：为了确定参与自主复制序列（ARS）功能的反式作用因子，我们启动了酿酒酵母突变体筛选工作，该突变体能够稳定含有缺陷ARS元件的质粒。在该筛选中恢复的amm（改变的微染色体维持）突变定义了至少四个互补组。已经详细研究了一种突变amm1，使一种有缺陷的ARS1质粒比野生型细胞中的水平稳定了17倍。该突变还影响至少一种带有野生型ARS元件的质粒的稳定性。 amm1是先前鉴定的TUP1基因的等位基因，与其他tup1突变体表现出相同的多效性表型。在携带TUP1基因破坏的菌株中，质粒的维持也受到影响。像amm1突变体一样，tup1破坏突变体也表现出ARS特异的质粒稳定作用。然而，这两个突变体的ARS特异性不同。第二位突变的恢复抑制了许多tup1表型，但没有增加质粒的维持性，这表明tup1突变体的质粒稳定性表型不是tup1引起的其他缺陷的结果。

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