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首页> 外文期刊>Molecular and Cellular Biology >The strong ADH1 promoter stimulates mitotic and meiotic recombination at the ADE6 gene of Schizosaccharomyces pombe.
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The strong ADH1 promoter stimulates mitotic and meiotic recombination at the ADE6 gene of Schizosaccharomyces pombe.

机译：强大的ADH1启动子可刺激粟酒裂殖酵母（Schizosaccharomyces pombe）ADE6基因的有丝分裂和减数分裂重组。

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摘要

The effect of the strong promoter from the alcohol dehydrogenase gene on mitotic and meiotic intragenic recombination has been studied at the ade6 locus of the fission yeast Schizosaccharomyces pombe. A 700-bp fragment containing the functional adh1 promoter was used to replace the weak wild-type promoter of the ade6 gene. Analysis of mRNA showed that strains with this ade6::adh1 fusion construct had strongly elevated ade6-specific mRNA levels during vegetative growth as well as in meiosis. These increased levels of mRNA correlated with a 20- to 25-fold stimulation of intragenic recombination in meiosis and a 7-fold increased prototroph formation during vegetative growth. Analysis of flanking marker configurations of prototrophic recombinants indicated that simple conversions as well as conversions associated with crossing over were stimulated in meiosis. The strongest stimulation of recombination was observed when the adh1 promoter was homozygous. Studies with heterologous promoter configurations revealed that the highly transcribed allele was the preferred acceptor of genetic information. The effect of the recombinational hot spot mutation ade6-M26 was also investigated in this system. Its effect was only partly additive to the elevated recombination rate generated by the ade6::adh1 fusion construct.
机译：在裂殖酵母粟酒裂殖酵母的ade6基因座上研究了乙醇脱氢酶基因的强启动子对有丝分裂和减数分裂基因内重组的影响。包含功能性adh1启动子的700 bp片段用于替换ade6基因的弱野生型启动子。对mRNA的分析表明，具有这种ade6 :: adh1融合构建体的菌株在营养生长以及减数分裂过程中，其ade6特异性mRNA的水平都大大升高。这些增加的mRNA水平与减数分裂中基因内重组的20到25倍刺激和营养生长期间原养型形成增加7倍相关。对原养重组体的侧翼标记构型的分析表明，减数分裂中刺激了简单的转化以及与交叉相关的转化。当adh1启动子是纯合子时，观察到最强烈的重组刺激。具有异源启动子构型的研究表明，高度转录的等位基因是遗传信息的首选受体。在该系统中还研究了重组热点突变ade6-M26的作用。它的作用只是部分地增加了ade6 :: adh1融合构建体产生的重组率。

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