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首页> 外文期刊>Molecular and Cellular Biology >Isolation and characterization of PKC-L, a new member of the protein kinase C-related gene family specifically expressed in lung, skin, and heart.
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Isolation and characterization of PKC-L, a new member of the protein kinase C-related gene family specifically expressed in lung, skin, and heart.

机译：PKC-L的分离和鉴定，PKC-L是蛋白激酶C相关基因家族的新成员，在肺，皮肤和心脏中特异性表达。

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摘要

We have isolated and characterized a new human cDNA, coding for a protein kinase, related to the protein kinase C (PKC) gene family. Although this protein kinase shares some homologous sequences and structural features with the four members of the PKC family initially isolated (alpha, beta I, beta II, and gamma), it shows more homology with the recently described PKC-related subfamily, encoded by the cDNAs delta, epsilon, and zeta. The transcript for this gene product, termed PKC-L, is most abundant in lung tissue, less expressed in heart and skin tissue, and exhibited very low expression in brain tissue. Thus, its tissue distribution is different from that described for other mammalian members of the PKC gene family, their expression being enriched in brain tissues. PKC-L is also expressed in several human cell lines, including the human epidermoid carcinoma line A431. The ability of phorbol esters to bind to and stimulate the kinase activity of PKC-L was revealed by introducing the cDNA into COS cells.
机译：我们已经分离并鉴定了一个新的人类cDNA，编码一个与蛋白激酶C（PKC）基因家族有关的蛋白激酶。尽管此蛋白激酶与最初分离的PKC家族的四个成员（α，βI，βII和γ）具有某些同源序列和结构特征，但它与最近描述的由PKC编码的PKC相关亚家族具有更多的同源性。 cDNA三角洲，ε和zeta。该基因产物的转录本（称为PKC-L）在肺组织中含量最高，在心脏和皮肤组织中表达较少，而在脑组织中却表现出非常低的表达。因此，其组织分布不同于PKC基因家族其他哺乳动物成员所描述的组织分布，它们的表达富集于脑组织中。 PKC-L也在几种人细胞系中表达，包括人表皮样癌系A431。通过将cDNA导入COS细胞，可以揭示佛波酯与PKC-L结合并刺激其激酶活性的能力。

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