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首页> 外文期刊>Molecular and Cellular Biology >Three downstream sites repress transcription of a Ty2 retrotransposon in Saccharomyces cerevisiae.
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Three downstream sites repress transcription of a Ty2 retrotransposon in Saccharomyces cerevisiae.

机译：三个下游位点抑制酿酒酵母中Ty2逆转录转座子的转录。

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摘要

Transcription of Ty1 and Ty2 retrotransposons of the yeast Saccharomyces cerevisiae is modulated by multiple downstream regulatory sites. Both transposon families include a positively acting site within the transcribed region which resembles a higher eukaryotic enhancer. We have demonstrated the existence of a repression site distal to the enhancer of the Ty2-917 element. Here we describe experiments investigating the internal structure of this site. We show that this 200-bp region includes three distinct repression sites which we term DRSI (downstream repression site I), DRSII, and DRSIII. Individually each site causes almost twofold repression, and together the sites repress eightfold. Unexpectedly, when the entire region encompassing the DRS sites is moved outside the transcription unit, it acts as a qualitatively positively acting element. In this context the DRS sites still repress transcription, since eliminating them increases transcription further. That the region can activate transcription implies that it includes activation sites in addition to the three repression sites. The change from qualitatively negatively acting to positively acting must reflect a change in the relative effects of the multiple positive and negative sites; when moved outside the transcription unit, the activators predominate. Importantly, DRSII and DRSIII repress transcription autonomously when inserted upstream of a heterologous promoter activated by the transcriptional activator GCN4, showing that they are indeed transcriptional repression sites.
机译：酵母Saccharomyces cerevisiae的Ty1和Ty2逆转座子的转录受多个下游调控位点调控。两个转座子家族都在转录区域内包含一个正向作用位点，类似于一个较高的真核生物增强子。我们已经证明存在于Ty2-917元件增强子远端的阻抑位点。在这里，我们描述了调查该网站内部结构的实验。我们表明，这个200 bp的区域包括三个不同的阻抑位点，我们将其称为DRSI（下游阻抑位点I），DRSII和DRSIII。每个站点分别引起几乎两倍的抑制，而站点共同抑制八倍。出乎意料的是，当将包含DRS位点的整个区域移到转录单元之外时，它将充当定性的正作用元件。在这种情况下，DRS位点仍然抑制转录，因为消除它们会进一步增加转录。该区域可以激活转录意味着该区域除了三个阻抑位点之外还包括激活位点。从定性的负作用到正作用的变化必须反映出多个正负位点的相对作用的变化。当移到转录单元外时，激活子占主导地位。重要的是，当DRSII和DRSIII插入被转录激活因子GCN4激活的异源启动子的上游时，它会自主抑制转录，表明它们确实是转录抑制位点。

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