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首页> 外文期刊>Molecular and Cellular Biology >Casein kinase I-like protein kinases encoded by YCK1 and YCK2 are required for yeast morphogenesis.
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Casein kinase I-like protein kinases encoded by YCK1 and YCK2 are required for yeast morphogenesis.

机译：YCK1和YCK2编码的酪蛋白激酶I样蛋白激酶是酵母形态发生所必需的。

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摘要

Casein kinase I is an acidotropic protein kinase class that is widely distributed among eukaryotic cell types. In the yeast Saccharomyces cerevisiae, the casein kinase I isoform encoded by the gene pair YCK1 and YCK2 is a 60- to 62-kDa membrane-associated form. The Yck proteins perform functions essential for growth and division; either alone supports growth, but loss of function of both is lethal. We report here that casein kinase I-like activity is associated with a soluble Yck2-beta-galactosidase fusion protein in vitro and that thermolabile protein kinase activity is exhibited by a protein encoded by fusion of a temperature-sensitive yck2 allele with lacZ. Cells carrying the yck2-2ts allele arrest at restrictive temperature with multiple, elongated buds containing multiple nuclei. This phenotype suggests that the essential functions of the Yck proteins include roles in bud morphogenesis, possibly in control of cell growth polarity, and in cytokinesis or cell separation. Further, a genetic relationship between the yck2ts allele and deletion of CDC55 indicates that the function of Yck phosphorylation may be related to that of protein phosphatase 2A activity.
机译：酪蛋白激酶I是一种嗜酸性蛋白激酶，在真核细胞类型中广泛分布。在酵母酿酒酵母中，由基因对YCK1和YCK2编码的酪蛋白激酶I亚型为60-62kDa的膜相关形式。 Yck蛋白执行生长和分裂所必需的功能;两者都单独支持生长，但是两者的功能丧失都是致命的。我们在这里报告酪蛋白激酶I样活性与体外可溶性Yck2-β-半乳糖苷酶融合蛋白相关，并且热不稳定性蛋白激酶活性由温度敏感的yck2等位基因与lacZ融合编码的蛋白表现出来。携带yck2-2ts等位基因的细胞在限制性温度下被多个带有多个核的细长芽抑制。此表型表明，Yck蛋白的基本功能包括在芽形态发生中的作用，可能在控制细胞生长极性以及胞质分裂或细胞分离中起作用。此外，yck2ts等位基因与CDC55缺失之间的遗传关系表明，Yck磷酸化的功能可能与蛋白磷酸酶2A活性有关。

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