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首页> 外文期刊>Molecular and Cellular Biology >Displacement of an E-box-binding repressor by basic helix-loop-helix proteins: implications for B-cell specificity of the immunoglobulin heavy-chain enhancer.
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Displacement of an E-box-binding repressor by basic helix-loop-helix proteins: implications for B-cell specificity of the immunoglobulin heavy-chain enhancer.

机译：E-box结合阻遏物被基本的螺旋-环-螺旋蛋白置换：对免疫球蛋白重链增强剂B细胞特异性的影响。

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The activity of the immunoglobulin heavy-chain (IgH) enhancer is restricted to B cells, although it binds both B-cell-restricted and ubiquitous transcription factors. Activation of the enhancer in non-B cells upon overexpression of the basic helix-loop-helix (bHLH) protein E2A appears to be mediated not only by the binding of E2A to its cognate E box but also by the resulting displacement of a repressor from that same site. We have identified a "two-handed" zinc finger protein, denoted ZEB, the DNA-binding specificity of which mimics that of the cellular repressor. By employing a derivative E box that binds ZEB but not E2A, we have shown that the repressor is active in B cells and the IgH enhancer is silenced in the absence of binding competition by bHLH proteins. Hence, we propose that a necessary prerequisite of enhancer activity is the B-cell-specific displacement of a ZEB-like repressor by bHLH proteins.
机译：免疫球蛋白重链（IgH）增强子的活性仅限于B细胞，尽管它同时结合了B细胞限制性和普遍存在的转录因子。过度表达基本螺旋-环-螺旋（bHLH）蛋白E2A后，非B细胞中增强子的激活似乎不仅通过E2A与其同源E盒的结合来介导，而且还通过阻遏物从同一网站。我们已经鉴定出一种称为“ ZEB”的“双手”锌指蛋白，其DNA结合特异性可模仿细胞阻遏物。通过使用结合ZEB但不结合E2A的衍生E盒，我们显示了阻遏物在B细胞中具有活性，并且在没有bHLH蛋白的结合竞争的情况下，IgH增强子被沉默了。因此，我们提出增强子活性的必要先决条件是bHLH蛋白对ZEB样阻遏物的B细胞特异性置换。

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