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首页> 外文期刊>Molecular and Cellular Biology >The serum-inducible mouse gene Krox-24 encodes a sequence-specific transcriptional activator.
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The serum-inducible mouse gene Krox-24 encodes a sequence-specific transcriptional activator.

机译：血清诱导型小鼠基因Krox-24编码序列特异性转录激活因子。

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摘要

The mouse gene Krox-24 is transiently activated during cell cycle reentry. It encodes a protein with three zinc fingers similar to those of the transcription factor Sp1. Here we present a biochemical characterization of the gene products. Krox-24 mRNA is translated into two proteins of 82 and 88 kilodaltons, designated p82Krox-24 and p88Krox-24, respectively. p82Krox-24 is initiated at the first AUG codon of the open reading frame, whereas synthesis of p88Krox-24 starts at a non-AUG codon located upstream. Both proteins were synthesized in HeLa cells infected with recombinant vaccinia viruses expressing Krox-24 cDNAs. Under these conditions, they were found phosphorylated on serine residues and glycosylated. The availability of the proteins made possible the determination of the DNA recognition sequence. In vitro, Krox-24 bound specifically to the sequence 5'-GCG(C/G)GGGCG-3'. This sequence is similar but not identical to the Sp1 target sequence. Insertion of an oligomer for the binding site in cis, close to the herpes simplex virus thymidine kinase promoter, rendered this promoter responsive to Krox-24. Krox-24 is therefore a sequence-specific transcriptional activator. Krox-24-binding sites were found upstream of several serum-inducible genes, raising the possibility that Krox-24 is involved in the regulation of these genes.
机译：在细胞周期再进入过程中，小鼠基因Krox-24被瞬时激活。它编码具有三个锌指的蛋白质，类似于转录因子Sp1的锌指。在这里，我们介绍基因产物的生化特性。 Krox-24 mRNA被翻译为82和88道尔顿的两种蛋白质，分别称为p82Krox-24和p88Krox-24。 p82Krox-24在开放阅读框的第一个AUG密码子处起始，而p88Krox-24的合成在上游的非AUG密码子处起始。两种蛋白均在感染了表达Krox-24 cDNA的重组牛痘病毒的HeLa细胞中合成。在这些条件下，发现它们在丝氨酸残基上被磷酸化并被糖基化。蛋白质的可用性使得确定DNA识别序列成为可能。在体外，Krox-24特异性结合序列5'-GCG（C / G）GGGCG-3'。该序列与Sp1靶序列相似但不相同。靠近顺式单纯疱疹病毒胸苷激酶启动子的顺式结合位点的寡聚物的插入，使得该启动子对Krox-24有反应。因此，Krox-24是序列特异性转录激活因子。在几个血清诱导基因的上游发现了Krox-24结合位点，这增加了Krox-24参与这些基因调控的可能性。

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