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首页> 外文期刊>Molecular and Cellular Biology >Hyphal tip extension in Aspergillus nidulans requires the manA gene, which encodes phosphomannose isomerase.
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Hyphal tip extension in Aspergillus nidulans requires the manA gene, which encodes phosphomannose isomerase.

机译：在构巢曲霉中菌丝尖端延伸需要manA基因，该基因编码磷酸甘露糖异构酶。

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摘要

A strain of Aspergillus nidulans carrying a temperature-sensitive mutation in the manA gene produces cell walls depleted of D-mannose and forms hyphal tip balloons at the restrictive temperature (B.P. Valentine and B.W. Bainbridge, J. Gen. Microbiol. 109:155-168, 1978). We have isolated and characterized the manA gene and physically located it between 3.5 and 5.5 kb centromere distal of the riboB locus on chromosome VIII. The manA gene contains four introns and encodes a 50.6-kDa protein which has significant sequence identity to type I phosphomannose isomerase proteins from other eukaryotes. We have constructed by integrative transformation a null mutation in the manA gene which can only be maintained in a heterokaryotic strain with wild-type manA+ nuclei. Thus, a manA null mutation is lethal in A. nidulans. The phenotype of the mutation was analyzed in germinating conidia. Such conidia are able to commence germination but swell abnormally, sometimes producing a misshapen germ tube, before growth ceases. The reason for the lethality is probably the lack of synthesis of mannose-containing cell wall polymers that must be required for normal cell wall development in growing hyphae.
机译：在manA基因中携带温度敏感突变的构巢曲霉菌株在限定温度下产生耗尽D-甘露糖的细胞壁并形成菌丝尖端球囊（BP Valentine和BW Bainbridge，J.Gen.Microbiol.109：155-168 （1978年）。我们已经分离并鉴定了manA基因，并将其物理定位在染色体VIII上riboB基因座远端的3.5和5.5 kb着丝粒之间。 manA基因包含四个内含子，编码50.6-kDa蛋白，与来自其他真核生物的I型磷酸甘露糖异构酶蛋白具有明显的序列同一性。通过整合转化，我们构建了manA基因中的无效突变，该突变只能在具有野生型manA +核的异核菌株中得以维持。因此，manA无效突变在构巢曲霉中是致命的。在萌发的分生孢子中分析了突变的表型。这种分生孢子能够开始萌发，但会异常膨胀，有时会在生长停止之前产生畸形的胚芽管。致死性的原因可能是缺少合成的菌丝中正常细胞壁发育所必需的含甘露糖的细胞壁聚合物的合成。

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