C25, an essential RNA polymerase III subunit related to the RNA polymerase II subunit RPB7.

P P Sadhale; N A Woychik

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C25, an essential RNA polymerase III subunit related to the RNA polymerase II subunit RPB7.

机译：C25，一种与RNA聚合酶II亚基RPB7相关的必需RNA聚合酶III亚基。

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We identified a partially sequenced Saccharomyces cerevisiae gene which encodes a protein related to the S. cerevisiae RNA polymerase II subunit, RPB7. Several lines of evidence suggest that this related gene, YKL1, encodes the RNA polymerase III subunit C25. C25, like RPB7, is present in submolar ratios, easily dissociates from the enzyme, is essential for cell growth and viability, but is not required in certain transcription assays in vitro. YKL1 has ABF-1 and PAC upstream sequences often present in RNA polymerase subunit genes. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis mobility of the YKL1 gene product is equivalent to that of the RNA polymerase III subunit C25. Finally, a C25 conditional mutant grown at the nonpermissive temperature synthesizes tRNA at reduced rates relative to 5.8S rRNA, a hallmark of all characterized RNA polymerase III mutants.

机译：我们鉴定了部分测序的酿酒酵母基因，该基因编码与酿酒酵母RNA聚合酶II亚基RPB7相关的蛋白质。几条证据表明，这一相关基因YKL1编码RNA聚合酶III亚基C25。 C25和RPB7一样，以亚摩尔比存在，很容易从酶上解离，对于细胞生长和生存能力至关重要，但在某些体外转录试验中并不需要。 YKL1具有经常出现在RNA聚合酶亚基基因中的ABF-1和PAC上游序列。 YKL1基因产物的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳迁移率等同于RNA聚合酶III亚基C25的迁移率。最后，在非容许温度下生长的C25条件突变体以相对于5.8S rRNA降低的速率合成tRNA，5.8S rRNA是所有特征RNA聚合酶III突变体的标志。

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《Molecular and Cellular Biology》 |1994年第9期|共7页
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P P Sadhale; N A Woychik;
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中图分类细胞生物学;
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1. Subunit compositions of Arabidopsis RNA polymerases I and III reveal Pol I- and Pol III-specific forms of the AC40 subunit and alternative forms of the C53 subunit [J] . Ream Thomas S., Haag Jeremy R., Pontvianne Frederic, Nucleic Acids Research . 2015,第8期

机译：拟南芥RNA聚合酶I和III的亚基组成揭示了AC40亚基的Pol I和Pol III特异性形式以及C53亚基的替代形式
2. Subunit compositions of Arabidopsis RNA polymerases I and III reveal Pol I- and Pol III-specific forms of the AC40 subunit and alternative forms of the C53 subunit [J] . Angela D. Norbeck, Carrie D. Nicora, Craig S. Pikaard, Nucleic acids research . 2015,第8期

机译：拟南芥RNA聚合酶I和III的亚基组成揭示了AC40亚基的Pol I和Pol III特异性形式以及C53亚基的替代形式
3. Essential Roles of Bdp1, a Subunit of RNA Polymerase III Initiation Factor TFIIIB, in Transcription and tRNA Processing [J] . Akira Ishiguro, George A. Kassavetis, E. Peter Geiduschek Molecular and Cellular Biology . 2002,第10期

机译：Bdp1，RNA聚合酶III起始因子TFIIIB的一个亚基，在转录和tRNA加工中的基本作用。
4. Cell-free incorporation of photo-crosslinkable p-benzoyl-L-phenylalanine into the polymerase subunits α and ε of Escherichia coli DNA polymerase III [C] . Kiyoshi Ozawa, Gottfried Otting, Nicholas E. Dixon Incorporating of the Australian Society for Biochemistry and Molecular Biology Combio . 2009

机译：无细胞掺入光交联的p-苯甲酰基-1-苯丙氨酸进入聚合酶亚基α和ε的大肠杆菌DNA聚合酶III
5. Subunit compositions of Arabidopsis DNA-dependent RNA polymerases and the roles of the plant-specific RNA polymerases IV and V in gene silencing. [D] . Ream, Thomas Scott. 2009

机译：拟南芥DNA依赖性RNA聚合酶的亚基组成以及植物特异性RNA聚合酶IV和V在基因沉默中的作用。
6. C25 an essential RNA polymerase III subunit related to the RNA polymerase II subunit RPB7. [O] . P P Sadhale, N A Woychik 1994

机译：C25一种与RNA聚合酶II亚基RPB7相关的必需RNA聚合酶III亚基。
7. C25, an essential RNA polymerase III subunit related to the RNA polymerase II subunit RPB7. [O] . Sadhale, P P, Woychik, N A 1994

机译：C25，一种与RNA聚合酶II亚基RPB7相关的必需RNA聚合酶III亚基。

C25, an essential RNA polymerase III subunit related to the RNA polymerase II subunit RPB7.

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