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Subunit compositions of Arabidopsis DNA-dependent RNA polymerases and the roles of the plant-specific RNA polymerases IV and V in gene silencing.

机译：拟南芥DNA依赖性RNA聚合酶的亚基组成以及植物特异性RNA聚合酶IV和V在基因沉默中的作用。

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In addition to RNA polymerases I, II and III, the essential RNA polymerases present in all eukaryotes, plants have two additional nuclear RNA polymerases, abbreviated as Pol IV and Pol V, that play non-redundant roles in siRNA-directed DNA methylation and gene silencing. Using a combination of affinity purification and protein identification by mass spectrometry, my studies show that Arabidopsis Pol IV and Pol V are composed of subunits that are paralogous or identical to the twelve subunits of Pol II. Four subunits of Pol IV are distinct from their Pol II paralogs, six subunits of Pol V are distinct from their Pol II paralogs, and four subunits differ between Pol IV and Pol V. Importantly, the subunit differences occur in key positions relative to the template entry and RNA exit paths. My findings support the hypothesis that Pol IV and Pol V are Pol II-like enzymes that evolved specialized roles in the production of noncoding transcripts for RNA silencing and genome defense. In addition, I have determined the subunit compositions of Arabidopsis RNA polymerases I, II and III, providing a novel framework for comparative and functional analyses of subunits from all five DNA-dependent RNA polymerases in plants.

机译：除了RNA聚合酶I，II和III（所有真核生物中存在的必需RNA聚合酶）外，植物还具有两种其他的核RNA聚合酶，分别缩写为Pol IV和Pol V，它们在siRNA指导的DNA甲基化和基因中起非冗余作用沉默。通过结合使用亲和纯化和质谱鉴定蛋白质，我的研究表明拟南芥Pol IV和Pol V由与Pol II的十二个亚基同源或相同的亚基组成。 Pol IV的四个亚基与其Pol II旁系同源物不同，Pol V的六个亚基与Pol II旁系同源物不同，Pol IV和Pol V之间的四个亚基有所不同。重要的是，这些亚基的差异发生在相对于模板的关键位置进入和RNA退出路径。我的发现支持以下假设，即Pol IV和Pol V是类似于Pol II的酶，在产生非编码转录本以产生RNA沉默和基因组防御的过程中发挥了特殊作用。此外，我已经确定了拟南芥RNA聚合酶I，II和III的亚基组成，为比较和功能分析植物中所有五个DNA依赖性RNA聚合酶的亚基提供了新的框架。

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作者
Ream, Thomas Scott.;
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作者单位

Washington University in St. Louis.;

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授予单位 Washington University in St. Louis.;
学科 Biology Molecular.;Biology Genetics.
学位 Ph.D.
年度 2009
页码 430 p.
总页数 430
原文格式 PDF
正文语种 eng
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入库时间 2022-08-17 11:38:25

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1. 水稻叶绿体RNA聚合酶α亚基基因的克隆及结构分析 [J] . 孙崇荣, 杉浦昌弘植物学报：英文版 . 1989,第010期
2. Evolutionary connection between the catalytic subunits of DNA-dependent RNA polymerases and eukaryotic RNA-dependent RNA polymerases and the origin of RNA polymerases [J] . Lakshminarayan M Iyer, Eugene V Koonin, L Aravind BMC Structural Biology . 2003,第1期

机译：DNA依赖的RNA聚合酶和真核RNA依赖的RNA聚合酶的催化亚基之间的进化联系和RNA聚合酶的起源
3. Subunit compositions of Arabidopsis RNA polymerases I and III reveal Pol I- and Pol III-specific forms of the AC40 subunit and alternative forms of the C53 subunit [J] . Ream Thomas S., Haag Jeremy R., Pontvianne Frederic, Nucleic Acids Research . 2015,第8期

机译：拟南芥RNA聚合酶I和III的亚基组成揭示了AC40亚基的Pol I和Pol III特异性形式以及C53亚基的替代形式
4. Subunit compositions of Arabidopsis RNA polymerases I and III reveal Pol I- and Pol III-specific forms of the AC40 subunit and alternative forms of the C53 subunit [J] . Angela D. Norbeck, Carrie D. Nicora, Craig S. Pikaard, Nucleic acids research . 2015,第8期

机译：拟南芥RNA聚合酶I和III的亚基组成揭示了AC40亚基的Pol I和Pol III特异性形式以及C53亚基的替代形式
5. Mutation of RNA polymerase β-subunit confers in both enhancement of environmental bio re mediation and antibiotic production in a Streptomyces species [C] . S. Rachid International Conference on Petroleum and Mineral Resources . 2013

机译：RNA聚合酶β-亚基的突变赋予环境生物重新调解和抗生素生产的增强物种
6. The Origin and Evolution of Plant-Specific RNA Polymerases and Genes Involved in RNA-Directed DNA Methylation [D] . Trujillo, Joshua Tyler. 2019

机译：植物特异性RNA聚合酶的起源和演变和参与RNA定向的DNA甲基化的基因
7. Evolutionary connection between the catalytic subunits of DNA-dependent RNA polymerases and eukaryotic RNA-dependent RNA polymerases and the origin of RNA polymerases [O] . Lakshminarayan M Iyer, Eugene V Koonin, L Aravind 2003

机译：DNA依赖的RNA聚合酶和真核RNA依赖的RNA聚合酶的催化亚基之间的进化联系和RNA聚合酶的起源
8. Evolutionary connection between the catalytic subunits of DNA-dependent RNA polymerases and eukaryotic RNA-dependent RNA polymerases and the origin of RNA polymerases [O] . Iyer Lakshminarayan M, Koonin Eugene V, Aravind L 2003

机译：DNA依赖的RNA聚合酶和真核RNA依赖的RNA聚合酶的催化亚基之间的进化联系和RNA聚合酶的起源

Subunit compositions of Arabidopsis DNA-dependent RNA polymerases and the roles of the plant-specific RNA polymerases IV and V in gene silencing.

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