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首页> 外文期刊>Molecular and Cellular Biology >Structure of REC2, a recombinational repair gene of Ustilago maydis, and its function in homologous recombination between plasmid and chromosomal sequences.
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Structure of REC2, a recombinational repair gene of Ustilago maydis, and its function in homologous recombination between plasmid and chromosomal sequences.

机译：REC2（玉米粉虱的重组修复基因）的结构及其在质粒和染色体序列之间的同源重组中的功能。

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摘要

Mutation in the REC2 gene of Ustilago maydis leads to defects in DNA repair, recombination, and meiosis. Analysis of the primary sequence of the Rec2 protein reveals a region with significant homology to bacterial RecA protein and to the yeast recombination proteins Dmc1, Rad51, and Rad57. This homologous region in the U. maydis Rec2 protein was found to be functionally sensitive to mutation, lending support to the hypothesis that Rec2 has a functional RecA-like domain essential for activity in recombination and repair. Homologous recombination between plasmid and chromosomal DNA sequences is reduced substantially in the rec2 mutant following transformation. The frequency can be restored to a level approaching, but not exceeding, that observed in the wild-type strain if transformation is performed with cells containing multiple copies of REC2.
机译：Ustilago REC2基因的突变可能导致DNA修复，重组和减数分裂的缺陷。对Rec2蛋白的一级序列的分析揭示了与细菌RecA蛋白以及酵母重组蛋白Dmc1，Rad51和Rad57具有显着同源性的区域。发现U. maydis Rec2蛋白中的该同源区域对突变功能敏感，从而为Rec2具有重组和修复活性必不可少的功能性RecA样结构域的假设提供了支持。转化后rec2突变体中质粒和染色体DNA序列之间的同源重组显着降低。如果使用包含多个REC2副本的细胞进行转化，则频率可以恢复到接近但不超过野生型菌株中观察到的水平。

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