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首页> 外文期刊>Molecular and Cellular Biology >Saccharomyces cerevisiae spheroplasts are sensitive to the action of diphtheria toxin.
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Saccharomyces cerevisiae spheroplasts are sensitive to the action of diphtheria toxin.

机译：酿酒酵母原生质球对白喉毒素的作用敏感。

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摘要

Diphtheria toxin kills spheroplasts of Saccharomyces cerevisiae but not the intact yeast cells. After 2 h of exposure to ca. 10(-7) M toxin, less than 1% of spheroplasts were able to regenerate into intact cells. The same high levels of toxin inhibited the rate of protein synthesis by more than 90% within 1 h, whereas RNA and DNA synthesis were not inhibited until 4 h or exposure. Both killing and protein synthesis inhibition were dependent on toxin concentration. The nature of the toxin-cell interaction was also studied by using fragments of intact toxin and mutant toxin proteins. Neither toxin fragment A nor CRM45 nor CRM197 affected spheroplasts, but CRM197 and ATP prevented the inhibitory action of intact toxin. These results suggest that toxin acts on S. cerevisiae spheroplasts in much the same manner as it acts on sensitive mammalian cells.
机译：白喉毒素可杀死酿酒酵母的原生质球，但不会杀死完整的酵母细胞。暴露于约2小时后。 10（-7）M毒素中，不到1％的原生质球能够再生为完整细胞。同样高水平的毒素在1小时内抑制了90％以上的蛋白质合成率，而RNA和DNA合成直到4小时或暴露才被抑制。杀伤和蛋白质合成抑制均取决于毒素浓度。还通过使用完整毒素和突变毒素蛋白的片段研究了毒素-细胞相互作用的性质。毒素片段A，CRM45或CRM197均不影响原生质球，但CRM197和ATP不能阻止完整毒素的抑制作用。这些结果表明，毒素对酿酒酵母原生质球的作用与其对敏感哺乳动物细胞的作用几乎相同。

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