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首页> 外文期刊>Molecular and Cellular Biology >Inhibition of vimentin synthesis and disruption of intermediate filaments in simian virus 40-infected monkey kidney cells.
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Inhibition of vimentin synthesis and disruption of intermediate filaments in simian virus 40-infected monkey kidney cells.

机译：猿猴病毒40感染的猴肾细胞中波形蛋白合成的抑制和中间丝的破坏。

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The organization, synthesis, and phosphorylation of vimentin were studied at various times after infection of monkey kidney cells with simian virus 40. Late after infection (between 36 and 48 h postinfection) there is a dramatic reduction in vimentin synthesis that is paralleled by a specific disruption of the intermediate filament network. At the same time there is no apparent alteration of the organization or the synthesis of the actin-containing filaments and of the microtubules. The inhibition of vimentin synthesis is also reflected by the level of vimentin mRNA activity in the infected cells, as assayed in a cell-free in vitro translation system, and vimentin mRNA concentration as revealed by RNA blot hybridization to cloned vimentin cDNA. The level of vimentin phosphorylation also decreases dramatically but at a much earlier time after infection (between 14 and 24 h postinfection), when mitosis in the infected cells is blocked. Although the decrease in vimentin synthesis in simian virus 40-infected cells is paralleled by the alterations in the organization of the intermediate filament network, the phosphorylation of vimentin correlates with the cell cycle, as it does in other systems. A possible feedback control mechanism of vimentin synthesis by alterations in the organization of the intermediate filament network is discussed.
机译：在猴肾细胞被猿猴病毒40感染后的不同时间，研究了波形蛋白的组织，合成和磷酸化。感染后期（感染后36至48小时之间）波形蛋白的合成显着减少，这与特异性中间丝网络的破坏。同时，含肌动蛋白的细丝和微管的组织或合成没有明显改变。在无细胞体外翻译系统中测定，波形蛋白合成的抑制还反映在感染细胞中波形蛋白mRNA的活性水平上，以及通过与克隆的波形蛋白cDNA进行RNA印迹杂交显示波形蛋白mRNA的浓度。波形蛋白磷酸化水平也显着降低，但是在感染后的更早时间（感染后14至24小时之间），此时感染细胞的有丝分裂被阻断了。尽管猿猴病毒40感染的细胞中波形蛋白合成的减少与中间丝网络的组织结构的变化平行，但波形蛋白的磷酸化与细胞周期相关，就像在其他系统中一样。讨论了波形蛋白合成的一种可能的反馈控制机制，该机制通过改变中间丝网络的组织而实现。

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