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首页> 外文期刊>Molecular and Cellular Biology >Linkage of the MBG locus to another functionally hemizygous gene locus (IDH2) on chromosome Z3 in Chinese hamster ovary cells.
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Linkage of the MBG locus to another functionally hemizygous gene locus (IDH2) on chromosome Z3 in Chinese hamster ovary cells.

机译：MBG基因座与中国仓鼠卵巢细胞Z3染色体上另一个功能性半合基因基因座（IDH2）的链接。

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To gain insight into the nature of hemizygosity in Chinese hamster ovary (CHO) cells and the mechanisms by which it has arisen, we are attempting to map and determine linkage relationships for as many hemizygous loci as possible. In this study, we have shown by segregation analysis of intraspecific somatic cell hybrids that the hemizygous gene locus associated with resistance to methylglyoxalbisguanyl hydrazone (MBG) in CHO cells is linked to the hemizygous IDH2 locus on chromosome Z3. Nine of the ten autosomal hemizygous gene loci that have been mapped to date in CHO cells are clustered on three chromosomes, with five such markers on chromosome 2, two on chromosome 8, and now two on the Z3 chromosome. With the mapping of MBG to the Z3 chromosome, selectable drug resistance markers are now available on eight different CHO chromosomes.
机译：为了深入了解中国仓鼠卵巢（CHO）细胞中半合子的性质及其产生的机制，我们尝试绘制和确定尽可能多的半合子基因座的连锁关系。在这项研究中，我们通过种内体细胞杂交体的分离分析显示，与CHO细胞中对甲基乙二醛双胍guan（MBG）的抗性相关的半合子基因位点与Z3染色体上的半合子IDH2位点相关。迄今为止，已在CHO细胞中定位的10个常染色体半合子基因座中有9个聚集在三个染色体上，其中5个这样的标记位于2号染色体上，两个位于8号染色体上，现在两个位于Z3染色体上。通过将MBG映射到Z3染色体，现在可以在八种不同的CHO染色体上使用可选的耐药标记。

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