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首页> 外文期刊>Molecular and Cellular Biology >Amino acid substitutions sufficient to convert the nontransforming p60c-src protein to a transforming protein.
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Amino acid substitutions sufficient to convert the nontransforming p60c-src protein to a transforming protein.

机译：足以将非转化性p60c-src蛋白转化为转化蛋白的氨基酸取代。

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We have previously shown that Rous sarcoma virus variants that carry the cellular homolog (c-src) of the viral src gene (v-src) do not transform chicken embryo fibroblasts. We also have shown that replacement of sequences upstream or downstream from the BglI site of the cellular src gene with the corresponding regions of v-src restored transforming activity to the hybrid genes. Since there are only six amino acid changes between p60c-src and p60v-src within the sequences upstream from BglI, we constructed chimeric molecules involving v-src and c-src to determine the effect of each amino acid substitution on the biological activities of the gene product. We found that the change from Thr to Ile at position 338 or the replacement of a fragment of c-src containing Gly-63, Arg-95, and Thr-96 with a corresponding fragment of v-src containing Asp-63, Trp-95, and Ile-96 converted p60c-src into a transforming protein by the criteria of focus formation, anchorage-independent growth, and tumor formation in newborn chickens. These mutations also resulted in elevation of the protein kinase activity of p60c-src.
机译：先前我们已经表明，携带病毒src基因（v-src）的细胞同源物（c-src）的劳斯肉瘤病毒变体不会转化鸡胚成纤维细胞。我们还已经表明，用v-src的相应区域替换细胞src基因的BglI位点上游或下游的序列，恢复了向杂种基因的转化活性。由于BglI上游序列中p60c-src和p60v-src之间只有六个氨基酸变化，因此我们构建了涉及v-src和c-src的嵌合分子，以确定每个氨基酸取代对BglI生物学活性的影响。基因产物。我们发现，在位置338处从Thr变为Ile或将包含Gly-63，Arg-95和Thr-96的c-src片段替换为包含Asp-63，Trp-的v-src片段95，和Ile-96按照新生鸡的病灶形成，不依赖锚定的生长和肿瘤形成的标准将p60c-src转化为转化蛋白。这些突变还导致p60c-src的蛋白激酶活性升高。

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