...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Mouse apolipoprotein A-IV gene: nucleotide sequence and induction by a high-lipid diet.
【24h】

Mouse apolipoprotein A-IV gene: nucleotide sequence and induction by a high-lipid diet.

机译：小鼠载脂蛋白A-IV基因：核苷酸序列和高脂饮食诱导。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Apolipoprotein A-IV (apo A-IV) functions in conjunction with other apolipoproteins to form lipoprotein particles which are involved in lipid homeostasis. In this report we present the nucleotide sequence of the mouse apo A-IV gene and demonstrate its induction in the liver by chronically high dietary lipid. The apo A-IV gene consists of three exons and two introns. The introns separate evolutionarily conserved and functional polypeptide domains. Intron 1 divides most of the apo A-IV signal peptide from the amino terminus of the mature plasma protein. The second intron separates a highly evolutionarily conserved, variant amphipathic peptide repeat from the remainder of the mature apo A-IV protein. The 5' flanking region has several interesting features. The apo A-IV gene has variant TATA and CAT box sequences, TTTAAA and CCAACG, respectively. There are five G-rich direct repeats of 10 nucleotides and a short inverted repeat in the 5' flanking region. We speculate that these sequence elements in the 5' flanking region may be involved in the regulation of apo A-IV gene expression. We also show that chronically high dietary lipid induces liver apo A-IV levels 10-fold in C57BL/6 mice, a strain susceptible to atherosclerotic lesions, while we observed no induction in nonsusceptible BALB/c and C3H mice.
机译：载脂蛋白A-IV（apo A-IV）与其他载脂蛋白一起形成脂蛋白颗粒，参与脂质体内平衡。在此报告中，我们介绍了小鼠载脂蛋白A-IV基因的核苷酸序列，并证明了其通过长期高饮食脂质在肝脏中的诱导作用。载脂蛋白A-IV基因由三个外显子和两个内含子组成。内含子将进化保守和功能性多肽结构域分开。内含子1从成熟血浆蛋白的氨基末端分离出大部分apo A-IV信号肽。第二个内含子从成熟的载脂蛋白A-IV蛋白的其余部分中分离出高度进化保守的变异两亲性肽重复序列。 5'侧翼区域具有一些有趣的功能。载脂蛋白A-IV基因分别具有变体TATA和CAT盒序列，TTTAAA和CCAACG。在5'侧翼区域有5个10个核苷酸的富含G的直接重复序列和一个短的反向重复序列。我们推测5'侧翼区域中的这些序列元件可能参与对载脂蛋白A-IV基因表达的调节。我们还显示，长期高饮食脂诱导C57BL / 6小鼠（易患动脉粥样硬化病变的菌株）的肝载脂蛋白A-IV水平提高了10倍，而我们未观察到在不敏感的BALB / c和C3H小鼠中没有诱导作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号