A recessive cellular mutation in v-fes-transformed mink cells restores contact inhibition and anchorage-dependent growth.

J R Haynes; J R Downing

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A recessive cellular mutation in v-fes-transformed mink cells restores contact inhibition and anchorage-dependent growth.

机译：v-fes转化的水貂细胞中的隐性细胞突变可恢复接触抑制和锚定依赖性生长。

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A contact-inhibited revertant of mink cells transformed by the Gardner-Arnstein strain of feline sarcoma virus was isolated by fluorescence-activated sorting of cells stained with the mitochondria-specific dye rhodamine 123. The revertant cell line exhibited a decrease in its proliferative rate and saturation density and a complete loss of its capacity for anchorage-independent growth, but it remained tumorigenic when inoculated into nude mice. The revertant cells retained a rescuable Gardner-Arnstein feline sarcoma provirus, expressed high levels of the v-fes oncogene product and its associated tyrosine kinase activity, manifested elevated levels of phosphotyrosine-containing cellular proteins similar to those observed in v-fes-transformed cells, and were refractory to retransformation by retroviruses containing the v-fes, v-fms, and v-ras oncogenes. Fusion of the revertant and parental cells generated somatic cell hybrids which formed colonies in semisolid medium, indicating that the block in transformation was recessive. These data together with the observation that the revertant phenotype is unstable in continuous culture suggest that the loss of transformation is due to the presence of limiting quantities of a gene product which functions downstream of the v-fes-coded kinase in the mitogenic pathway.

机译：通过线粒体特异性染料若丹明123染色的细胞的荧光激活分选，分离了由猫恶性肉瘤病毒Gardner-Arnstein株转化的水貂细胞的接触抑制逆转株。该逆转株细胞系的增殖率和饱和密度和其完全不依赖锚固生长的能力完全丧失，但接种裸鼠后仍保持致癌性。回复细胞保留了可挽救的Gardner-Arnstein猫肉瘤肉瘤原病毒，表达了高水平的v-fes癌基因产物及其相关的酪氨酸激酶活性，表现出与经v-fes转化的细胞相似的含磷酸酪氨酸的细胞蛋白水平升高，并且对含有v-fes，v-fms和v-ras癌基因的逆转录病毒难以转化。回复性细胞和亲代细胞的融合产生了体细胞杂种，其在半固体培养基中形成了集落，表明转化的障碍是隐性的。这些数据以及在连续培养中可逆的表型不稳定的观察结果表明，转化的损失是由于在有丝分裂途径中在v-fes编码的激酶的下游起作用的有限量的基因产物的存在所致。

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《Molecular and Cellular Biology》 |1988年第6期|共9页
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J R Haynes; J R Downing;
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中图分类细胞生物学;
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6. A recessive cellular mutation in v-fes-transformed mink cells restores contact inhibition and anchorage-dependent growth. [O] . J R Haynes, J R Downing 1988

机译：v-fes转化的水貂细胞中的隐性细胞突变可恢复接触抑制和锚定依赖性生长。
7. A recessive cellular mutation in v-fes-transformed mink cells restores contact inhibition and anchorage-dependent growth. [O] . Haynes, J R, Downing, J R 1988

机译：v-fes转化的水貂细胞中的隐性细胞突变可恢复接触抑制和锚定依赖性生长。

A recessive cellular mutation in v-fes-transformed mink cells restores contact inhibition and anchorage-dependent growth.

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