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首页> 外文期刊>Molecular and Cellular Biology >gcr2, a new mutation affecting glycolytic gene expression in Saccharomyces cerevisiae.
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gcr2, a new mutation affecting glycolytic gene expression in Saccharomyces cerevisiae.

机译：gcr2，一种新突变，影响酿酒酵母中糖酵解基因的表达。

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Screening of a mutagenized strain carrying a multicopy ENO1-'lacZ fusion plasmid revealed a new mutation affecting most glycolytic enzyme activities in a pattern resembling that caused by gcr1: levels in the range of 10% of wild-type levels on glycerol plus lactate but somewhat higher on glucose. The recessive single nuclear gene mutation, named gcr2-1, was unlinked to gcr1, and GCR1 in multiple copies did not restore enzyme levels. GCR2 was obtained by complementation from a YCp50 genomic library; the complemented strain had normal enzyme levels, as did a strain with GCR2 in multiple copies. GCR2 in multiple copies did not suppress gcr1. A chromosomal gcr2 null mutant was constructed; its pattern of enzyme activities resembled that of the gcr2-1 mutant and, like the gcr2-1 mutant, its growth defect on glucose was only partial (in contrast to the glucose negativity of the gcr1 mutant). Northern (RNA) analysis showed that gcr2 and gcr1 affect ENO1 mRNA levels.
机译：筛选携带多拷贝ENO1-'lacZ融合质粒的诱变菌株，发现了一个新突变，该突变以gcr1引起的模式影响了大多数糖酵解酶的活性：甘油和乳酸的野生型水平在10％范围内，但有些葡萄糖含量更高。隐性单核基因突变，称为gcr2-1，未与gcr1关联，并且多个拷贝的GCR1不能恢复酶水平。 GCR2通过从YCp50基因组文库互补获得。互补菌株的酶水平正常，多拷贝GCR2菌株也是如此。多份GCR2不能抑制gcr1。构建了染色体gcr2无效突变体。其酶活性模式与gcr2-1突变体相似，并且与gcr2-1突变体一样，其在葡萄糖上的生长缺陷仅是部分的（与gcr1突变体的葡萄糖负性相反）。 Northern（RNA）分析显示gcr2和gcr1影响ENO1 mRNA水平。

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