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首页> 外文期刊>Yeast >Influence of low glycolytic activities in gcr1 and gcr2 mutants on the expression of other metabolic pathway genes in Saccharomyces cerevisiae
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Influence of low glycolytic activities in gcr1 and gcr2 mutants on the expression of other metabolic pathway genes in Saccharomyces cerevisiae

机译：gcr1和gcr2突变体中低糖酵解活性对酿酒酵母中其他代谢途径基因表达的影响

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摘要

A complex of the transcription factors Gcr1p and Gcr2p coordinately regulates the expression of glycolytic genes in Saccharomyces cerevisiae. To understand the effects of gcr mutations on other metabolic pathways, genome-wide gene expression profiles in gcr1 and gcr2 mutants were examined. The biggest effects of gcr1 and gcr2 mutations were observed on the glycolytic genes and the expressions of most of the glycolytic genes were substantially decreased compared to those in the wild-type strain in both glucose and glycerol+lactate growth conditions. On the other hand, the expressions of genes encoding the TCA cycle and respiration were increased in gcr mutants when the cells were grown in glucose. RT-PCR analyses revealed that the expression of SIP4 and HAP5, which are known to affect the expression of some of the gluconeogenic, TCA cycle and respiratory genes, were also increased under this condition. The growth of gcr mutants on glucose was impaired by adding respiration inhibitor antimycin A, whereas the growth of the wild-type strain was not. The conversion of glucose to biomass was higher and, to the contrary, ethanol yield was lower in the gcr2 mutant compared to those in the wild-type strain. These results suggest the possibility that the gcr mutants, in which glycolytic activities are low, changed their metabolic patterns under glucose growth condition to enhance the expression of TCA cycle and respiratory genes to produce more energy.
机译：转录因子Gcr1p和Gcr2p的复合物可协调调节酿酒酵母中糖酵解基因的表达。为了了解gcr突变对其他代谢途径的影响，检查了gcr1和gcr2突变体的全基因组基因表达谱。观察到gcr1和gcr2突变对糖酵解基因的最大影响，并且在葡萄糖和甘油+乳酸生长条件下，与野生型菌株相比，大多数糖酵解基因的表达均显着降低。另一方面，当细胞在葡萄糖中生长时，gcr突变体中编码TCA循环和呼吸的基因的表达增加。 RT-PCR分析显示，在这种情况下，已知会影响某些糖原异生性，TCA周期和呼吸基因表达的SIP4和HAP5的表达也有所增加。通过添加呼吸抑制剂抗霉素A来削弱gcr突变体在葡萄糖上的生长，而野生型菌株的生长却没有。与野生型菌株相比，gcr2突变体中葡萄糖向生物质的转化率更高，相反，乙醇产率较低。这些结果表明，糖酵解活性低的gcr突变体在葡萄糖生长条件下改变其代谢模式以增强TCA循环和呼吸基因表达以产生更多能量的可能性。

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