Erythroid-specific nuclease-hypersensitive sites flanking the human beta-globin domain.

V Dhar; A Nandi; C L Schildkraut; A I Skoultchi

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Erythroid-specific nuclease-hypersensitive sites flanking the human beta-globin domain.

机译：人类β-珠蛋白结构域侧翼的类红细胞特异性核酸酶超敏位点。

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Recent evidence suggests that DNA sequences from the region lying 5' of the human epsilon-globin gene are important for erythroid-specific expression of human beta-like globin genes. This region, as well as a region 20 kilobases (kb) downstream from the beta-globin gene, contains a set of developmentally stable, DNase I-superhypersensitive sites that are thought to reflect a chromatin structure supporting active globin gene expression. We have analyzed the chromatin structure in these two regions in a wide variety of nonerythroid and erythroid cells. The study included analysis of chromatin structure changes occurring during globin gene activation in mouse erythroleukemia-human nonerythroid cell hybrids. The results identified a hypersensitive site (III) 14.8 kb upstream of the epsilon-globin gene that was strictly correlated with active globin gene transcription. Interestingly, a multipotent human embryonal carcinoma cell line exhibited a hypersensitive site (IV) 18.4 kb upstream of epsilon-globin that was absent in all other nonerythroid cells examined, suggesting that chromatin structure changes at specific hypersensitive sites during embryonic development may also be important in globin gene repression.

机译：最近的证据表明，来自人类ε-球蛋白基因5'区域的DNA序列对于人类β-样球蛋白基因的红系特异性表达很重要。该区域以及β-珠蛋白基因下游20 kb（kb）的区域包含一组发育稳定的DNase I超高敏感位点，据认为该位点反映了支持活性珠蛋白基因表达的染色质结构。我们已经分析了多种非红系和红系细胞中这两个区域的染色质结构。该研究包括对小鼠红白血病-人非红系细胞杂种球蛋白基因激活过程中染色质结构变化的分析。结果确定了ε-珠蛋白基因上游14.8 kb的超敏位点（III），该位点与活性珠蛋白基因的转录严格相关。有趣的是，多能人胚胎癌细胞系在所有检测到的非红系细胞中均不存在ε-珠蛋白上游18.4 kb的超敏位点（IV），这表明在胚胎发育过程中特定超敏位点的染色质结构变化可能也很重要。球蛋白基因抑制。

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《Molecular and Cellular Biology》 |1990年第8期|共10页
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V Dhar; A Nandi; C L Schildkraut; A I Skoultchi;
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中图分类细胞生物学;
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1. Conserved elements containing NF-E2 and tandem GATA binding sites are required for erythroid-specific chromatin structure reorganization within the human beta-globin locus control region. [J] . Pomerantz O, Goodwin AJ, Joyce T, Nucleic Acids Research . 1998,第24期

机译：在人β-珠蛋白基因座控制区域内，类胡萝卜素特异性染色质结构重组需要含有NF-E2和串联GATA结合位点的保守元件。
2. AUTONOMOUS, ERYTHROID-SPECIFIC DNASE I HYPERSENSITIVE SITE FORMED BY HUMAN BETA-GLOBIN LOCUS CONTROL REGION (LCR) 5' HS 2 IN TRANSGENIC MICE [J] . Pawlik KM., Townes TM. Developmental biology . 1995,第2期

机译：人类β-球蛋白位置控制区（LCR）5'HS 2在转基因小鼠中形成的过敏性红斑狼疮特异性DNASE I
3. Chromatin structure at the flanking regions of the human beta-globin locus control region DNase I hypersensitive site-2: proposed nucleosome positioning by DNA-binding proteins including GATA-1 [J] . Davies N, Freebody J, Murray V Biochimica et biophysica acta. Gene structure and expression . 2004,第3期

机译：人类β球蛋白基因座控制区DNase I超敏位点2侧翼区域的染色质结构：建议的DNA结合蛋白（包括GATA-1）对核小体的定位
4. HUMAN EXPLORATION OF THE SOLAR SYSTEM SYMPOSIUM (A5):SITE SELECTION AND TRAVERSE PLANNING FOR HUMAN SORTIE MISSIONS: LESSONS LEARNED FROM ANALOGUE MISSIONS AT THE MISTASTIN LAKE IMPACT STRUCTURE,CANADA. Human Exploration of the Moon and Cislunar Space (1):SITE SELECTION AND TRAVERSE PLANNING FOR HUMAN SORTIE MISSIONS: LESSONS LEARNED FROM ANALOGUE MISSIONS AT THE MISTASTIN LAKE IMPACT STRUCTURE, CANADA. [C] . Bhairavi Shankar, Gordon Osinski, Anna Chanou, International Astronautical Congress . 2014

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5. Erythroid-specific expression of human growth hormone enhances trabecular bone formation along marrow -lining endosteal surfaces in transgenic mice [D] . Flay, Nina W. 2001

机译：人类生长激素的类胡萝卜素特异性表达可促进转基因小鼠沿衬有内膜的骨小梁表面的小梁骨形成
6. Erythroid-specific nuclease-hypersensitive sites flanking the human beta-globin domain. [O] . V Dhar, A Nandi, C L Schildkraut, 1990

机译：人类β-珠蛋白结构域侧翼的类红细胞特异性核酸酶超敏位点。
7. Erythroid-specific nuclease-hypersensitive sites flanking the human beta-globin domain. [O] . Dhar, V, Nandi, A, Schildkraut, C L, 1990

机译：人类β-珠蛋白结构域侧翼的类红细胞特异性核酸酶超敏位点。
8. Developmentally Stable Chromatin Structure in the Human Beta-Globin Gene Cluster [R] . Forrester, W. C., Thompson, C., Elder, J. T., 1986

机译：人β珠蛋白基因簇中发育稳定的染色质结构

Erythroid-specific nuclease-hypersensitive sites flanking the human beta-globin domain.

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