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首页> 外文期刊>Molecular and Cellular Biology >A yeast replicative helicase, Dna2 helicase, interacts with yeast FEN-1 nuclease in carrying out its essential function.
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A yeast replicative helicase, Dna2 helicase, interacts with yeast FEN-1 nuclease in carrying out its essential function.

机译：酵母复制解旋酶Dna2解旋酶在执行其基本功能时与酵母FEN-1核酸酶相互作用。

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摘要

We have recently described a new helicase, the Dna2 helicase, that is essential for yeast DNA replication. We now show that the yeast FEN-1 (yFEN-1) nuclease interacts genetically and biochemically with Dna2 helicase. FEN-1 is implicated in DNA replication and repair in yeast, and the mammalian homolog of yFEN-1 (DNase IV, FEN-1, or MF1) participates in Okazaki fragment maturation. Overproduction of yFEN-1, encoded by RAD27/RTH1, suppresses the temperature-sensitive growth of dna2-1 mutants. Overproduction of Dna2 suppresses the rad27/rth1 delta temperature-sensitive growth defect. dna2-1 rad27/rth1 delta double mutants are inviable, indicating that the mutations are synthetically lethal. The genetic interactions are likely due to direct physical interaction between the two proteins, since both epitope-tagged yFEN-1 and endogenous yFEN-1 coimmunopurify with tagged Dna2. The simplest interpretation of these data is that one of the roles of Dna2 helicase is associated with processing of Okazaki fragments.
机译：我们最近描述了一种新的解旋酶Dna2解旋酶，它对酵母DNA复制至关重要。我们现在显示酵母FEN-1（yFEN-1）核酸酶与Dna2解旋酶发生遗传和生化相互作用。 FEN-1与酵母中的DNA复制和修复有关，而yFEN-1的哺乳动物同源物（DNase IV，FEN-1或MF1）参与冈崎片段的成熟。 RAD27 / RTH1编码的yFEN-1的过量生产抑制了dna2-1突变体的温度敏感性生长。 Dna2的过量生产抑制了rad27 / rth1 delta温度敏感的生长缺陷。 dna2-1 rad27 / rth1 delta双突变体是不可行的，表明该突变是合成致死的。遗传相互作用可能是由于这两种蛋白质之间直接的物理相互作用，因为抗原表位标记的yFEN-1和内源性yFEN-1均与标记的Dna2共免疫纯化。这些数据的最简单解释是Dna2解旋酶的作用之一与Okazaki片段的加工有关。

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