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首页> 外文期刊>Molecular and Cellular Biology >Regulatory Interactions between the Reg1-Glc7 Protein Phosphatase and the Snf1 Protein Kinase
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Regulatory Interactions between the Reg1-Glc7 Protein Phosphatase and the Snf1 Protein Kinase

机译：Reg1-Glc7蛋白磷酸酶和Snf1蛋白激酶之间的调节相互作用。

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摘要

Protein phosphatase 1, comprising the regulatory subunit Reg1 and the catalytic subunit Glc7, has a role in glucose repression inSaccharomyces cerevisiae. Previous studies showed that Reg1 regulates the Snf1 protein kinase in response to glucose. Here, we explore the functional relationships between Reg1, Glc7, and Snf1. We show that different sequences of Reg1 interact with Glc7 and Snf1. We use a mutant Reg1 altered in the Glc7-binding motif to demonstrate that Reg1 facilitates the return of the activated Snf1 kinase complex to the autoinhibited state by targeting Glc7 to the complex. Genetic evidence indicated that the catalytic activity of Snf1 negatively regulates its interaction with Reg1. We show that Reg1 is phosphorylated in response to glucose limitation and that this phosphorylation requires Snf1; moreover, Reg1 is dephosphorylated by Glc7 when glucose is added. Finally, we show that hexokinase PII (Hxk2) has a role in regulating the phosphorylation state of Reg1, which may account for the effect of Hxk2 on Snf1 function. These findings suggest that the phosphorylation of Reg1 by Snf1 is required for the release of Reg1-Glc7 from the kinase complex and also stimulates the activity of Glc7 in promoting closure of the complex.
机译：包含调节亚基Reg1和催化亚基Glc7的蛋白磷酸酶1在酿酒酵母（Saccharomyces cerevisiae）中抑制葡萄糖的作用。以前的研究表明Reg1调节葡萄糖响应的Snf1蛋白激酶。在这里，我们探索Reg1，Glc7和Snf1之间的功能关系。我们显示Reg1的不同序列与Glc7和Snf1相互作用。我们使用在Glc7结合基序中更改的Reg1突变体来证明Reg1通过将Glc7靶向复合物来促进激活的Snf1激酶复合物返回到自动抑制状态。遗传证据表明，Snf1的催化活性负调节其与Reg1的相互作用。我们显示Reg1被响应葡萄糖限制磷酸化，并且此磷酸化需要Snf1;此外，当添加葡萄糖时，Reg1被Glc7去磷酸化。最后，我们显示己糖激酶PII（Hxk2）在调节Reg1的磷酸化状态中起作用，这可能解释了Hxk2对Snf1功能的影响。这些发现表明，Snf1使Reg1磷酸化是从激酶复合物中释放Reg1-Glc7所必需的，并且还刺激了Glc7促进复合物闭合的活性。

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