...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Serum Response Factor-GATA Ternary Complex Required for Nuclear Signaling by a G-Protein-Coupled Receptor
【24h】

Serum Response Factor-GATA Ternary Complex Required for Nuclear Signaling by a G-Protein-Coupled Receptor

机译：G蛋白偶联受体的核信号转导所需的血清反应因子-GATA三元复合物

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Endothelins are a family of biologically active peptides that are critical for development and function of neural crest-derived and cardiovascular cells. These effects are mediated by two G-protein-coupled receptors and involve transcriptional regulation of growth-responsive and/or tissue-specific genes. We have used the cardiac ANF promoter, which represents the best-studied tissue-specific endothelin target, to elucidate the nuclear pathways responsible for the transcriptional effects of endothelins. We found that cardiac-specific response to endothelin 1 (ET-1) requires the combined action of the serum response factor (SRF) and the tissue-restricted GATA proteins which bind over their adjacent sites, within a 30-bp ET-1 response element. We show that SRF and GATA proteins form a novel ternary complex reminiscent of the well-characterized SRF-ternary complex factor interaction required for transcriptional induction of c-fos in response to growth factors. In transient cotransfections, GATA factors and SRF synergistically activate atrial natriuretic factor and other ET-1-inducible promoters that contain both GATA and SRF binding sites. Thus, GATA factors may represent a new class of tissue-specific SRF accessory factors that account for muscle- and other cell-specific SRF actions.
机译：内皮素是一类生物活性肽，对神经that衍生和心血管细胞的发育和功能至关重要。这些作用是由两个G蛋白偶联受体介导的，涉及生长反应性和/或组织特异性基因的转录调控。我们已经使用了心脏ANF启动子（代表研究最好的组织特异性内皮素靶标）阐明负责内皮素转录作用的核途径。我们发现，对内皮素1（ET-1）的心脏特异性应答需要血清应答因子（SRF）和组织受限的GATA蛋白（在其30 bp ET-1应答中结合在其相邻位点上）的联合作用元件。我们表明，SRF和GATA蛋白形成了一种新颖的三元复合物，使人想起了对c- fos 的转录诱导响应于生长因子的特征明确的SRF-三元复合物相互作用。在瞬时共转染中，GATA因子和SRF协同激活心房利钠因子和其他同时包含GATA和SRF结合位点的ET-1诱导型启动子。因此，GATA因子可能代表一类新的组织特异性SRF辅助因子，可解释肌肉和其他细胞特异性SRF的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号