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首页> 外文期刊>Molecular and Cellular Biology >Saccharomyces cerevisiae BUR6 encodes a DRAP1/NC2alpha homolog that has both positive and negative roles in transcription in vivo.
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Saccharomyces cerevisiae BUR6 encodes a DRAP1/NC2alpha homolog that has both positive and negative roles in transcription in vivo.

机译：啤酒酵母BUR6编码DRAP1 / NC2alpha同源物，在体内转录中具有正作用和负作用。

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BUR3 and BUR6 were identified previously by selecting for mutations that increase transcription from an upstream activating sequence (UAS)-less promoter in Saccharomyces cerevisiae. The bur3-1 and bur6-1 mutations are recessive, increase transcription from a suc2 delta uas allele, and cause other mutant phenotypes, suggesting that Bur3p and Bur6p function as general repressors of the basal transcriptional machinery. The molecular cloning and characterization of BUR3 and BUR6 are presented here. BUR3 is identical to MOT1, a previously characterized essential gene that encodes an ATP-dependent inhibitor of the TATA box-binding protein. Cloning and nucleotide sequence analysis reveals that BUR6 encodes a homolog of DRAP1 (also called NC2alpha), a mammalian repressor of basal transcription. Strains that contain a bur6 null allele are viable but grow extremely poorly, demonstrating that BUR6 is critical for normal cell growth in yeast. The Bur6p histone fold domain is required for function; an extensive nonoverlapping set of deletion alleles throughout the histone fold domain impairs BUR6 function in vivo, whereas mutations in the amino- and carboxy-terminal tails have no detectable effect. BUR6 and BUR3/MOT1 have different functions depending on promoter context: although the bur3-1 and bur6-1 mutations increase transcription from delta uas promoters, they result in reduced transcription from the wild-type GAL1 and GAL10 promoters. This transcriptional defect is due to the inability of the GAL10 UAS to function in bur6-1 strains. The similar phenotypes of bur6 and bur3 (mot1) mutations suggest that Bur6p and Mot1p have related, but not identical, functions in modulating the activity of the general transcription machinery in vivo.
机译：BUR3和BUR6以前是通过在酿酒酵母中选择能增加无上游激活序列（UAS）启动子转录的突变来鉴定的。 bur3-1和bur6-1突变是隐性的，增加了suc2Δuas等位基因的转录，并引起其他突变表型，表明Bur3p和Bur6p充当了基础转录机制的一般阻遏物。这里介绍了BUR3和BUR6的分子克隆和表征。 BUR3与MOT1相同，MOT1是先前表征的必需基因，其编码TATA盒结合蛋白的ATP依赖性抑制剂。克隆和核苷酸序列分析表明，BUR6编码DRAP1（也称为NC2alpha）的同系物，DRAP1是基础转录的哺乳动物阻遏物。含有bur6无效等位基因的菌株是可行的，但生长极差，表明BUR6对酵母中正常细胞的生长至关重要。 Bur6p组蛋白折叠域是功能所必需的;整个组蛋白折叠结构域中广泛的不重叠的缺失等位基因集会削弱体内的BUR6功能，而氨基末端和羧基末端尾部的突变则没有可检测的作用。 BUR6和BUR3 / MOT1具有不同的功能，具体取决于启动子的背景：尽管bur3-1和bur6-1突变增加了Δuas启动子的转录，但它们导致野生型GAL1和GAL10启动子的转录减少。这种转录缺陷是由于GAL10 UAS无法在bur6-1菌株中发挥作用。 bur6和bur3（mot1）突变的相似表型表明Bur6p和Mot1p在调节体内一般转录机制的活性方面具有相关但不完全相同的功能。

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