• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Rok1p is a putative RNA helicase required for rRNA processing.
【24h】

Rok1p is a putative RNA helicase required for rRNA processing.

机译：Rok1p是rRNA加工所需的推定RNA解旋酶。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The synthesis of ribosomes involves many small nucleolar ribonucleoprotein particles (snoRNPs) as transacting factors. Yeast strains lacking the snoRNA, snR10, are viable but are impaired in growth and delayed in the early pre-rRNA cleavages at sites A0, A1, and A2, which lead to the synthesis of 18S rRNA. The same cleavages are inhibited by genetic depletion of the essential snoRNP protein Gar1p. Screens for mutations showing synthetic lethality with deletion of the SNR10 gene or with a temperature-sensitive gar1 allele both identified the ROK1 gene, encoding a putative, ATP-dependent RNA helicase of the DEAD-box family. The ROK1 gene is essential for viability, and depletion of Rok1p inhibits pre-rRNA processing at sites A0, A1, and A2, thereby blocking 18S rRNA synthesis. Indirect immunofluorescence by using a ProtA-Rok1p construct shows the protein to be predominantly nucleolar. These results suggest that Rok1p is required for the function of the snoRNP complex carrying out the early pre-rRNA cleavage reactions.
机译：核糖体的合成涉及许多小的核仁核糖核蛋白颗粒（snoRNPs）作为交易因子。缺少snoRNA，snR10的酵母菌株是可行的，但生长受到损害，并且在位点A0，A1和A2的早期rRNA早期裂解中被延迟，从而导致18S rRNA的合成。相同的切割被必需的snoRNP蛋白Gar1p的遗传消耗抑制。突变筛查显示出具有合成杀伤力，缺失SNR10基因或具有温度敏感性gar1等位基因的突变，均确定了ROK1基因，该基因编码DEAD-box家族推定的，依赖ATP的RNA解旋酶。 ROK1基因对于生存力至关重要，Rok1p的耗竭会抑制A0，A1和A2位点的前rRNA加工，从而阻止18S rRNA的合成。通过使用ProtA-Rok1p构建体进行的间接免疫荧光显示该蛋白质主要为核仁。这些结果表明，Rok1p是执行早期pre-rRNA裂解反应的snoRNP复合体功能所必需的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号