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首页> 外文期刊>Molecular and Cellular Biology >Dual Role of the Mitochondrial Chaperone Mdj1p in Inheritance of Mitochondrial DNA in Yeast
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Dual Role of the Mitochondrial Chaperone Mdj1p in Inheritance of Mitochondrial DNA in Yeast

机译：线粒体伴侣Mdj1p在酵母线粒体DNA遗传中的双重作用。

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摘要

Mdj1p, a homolog of the bacterial DnaJ chaperone protein, plays an essential role in the biogenesis of functional mitochondria in the yeast Saccharomyces cerevisiae. We analyzed the role of Mdj1p in the inheritance of mitochondrial DNA (mtDNA). Mitochondrial genomes were rapidly lost in a temperature-sensitive mdj1 mutant under nonpermissive conditions. The activity of mtDNA polymerase was severely reduced in the absence of functional Mdj1p at a nonpermissive temperature, demonstrating the dependence of the enzyme on Mdj1p. At a permissive temperature, the activity of mtDNA polymerase was not affected by the absence of Mdj1p. However, under these conditions, intact [rho +] genomes were rapidly converted to nonfunctional [rho ?] genomes which were stably propagated in an mdj1 deletion strain. We propose that mtDNA polymerase depends on Mdj1p as a chaperone in order to acquire and/or maintain an active conformation at an elevated temperature. In addition, Mdj1p is required for the inheritance of intact mitochondrial genomes at a temperature supporting optimal growth; this second function appears to be unrelated to the function of Mdj1p in maintaining mtDNA polymerase activity.
机译：Mdj1p是细菌DnaJ伴侣蛋白的同源物，在酵母的功能性线粒体的生物发生中起重要作用。我们分析了Mdj1p在线粒体DNA（mtDNA）遗传中的作用。在非允许条件下，对温度敏感的 mdj1 突变体中的线粒体基因组迅速丢失。在不允许的温度下，在不存在功能性Mdj1p的情况下，mtDNA聚合酶的活性严重降低，表明该酶对Mdj1p的依赖性。在允许的温度下，mtDNA聚合酶的活性不受Mdj1p缺失的影响。但是，在这些条件下，完整的[ rho + ]基因组迅速转换为无功能的[ rho ？]基因组在 mdj1 缺失株中稳定繁殖。我们建议mtDNA聚合酶依赖于Mdj1p作为伴侣，以便在升高的温度下获得和/或维持活性构象。另外，在支持最佳生长的温度下，完整的线粒体基因组的遗传需要Mdj1p。第二个功能似乎与Mdj1p在维持mtDNA聚合酶活性中的功能无关。

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