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首页> 外文期刊>Molecular and Cellular Biology >Candida albicans INT1-Induced Filamentation in Saccharomyces cerevisiae Depends on Sla2p
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Candida albicans INT1-Induced Filamentation in Saccharomyces cerevisiae Depends on Sla2p

机译：白色念珠菌INT1诱导的酿酒酵母中的长丝依赖于Sla2p。

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The Candida albicans INT1 gene is important for hyphal morphogenesis, adherence, and virulence (C. Gale, C. Bendel, M. McClellan, M. Hauser, J. M. Becker, J. Berman, and M. Hostetter, Science 279:1355–1358, 1998). The ability to switch between yeast and hyphal morphologies is an important virulence factor in this fungal pathogen. When INT1 is expressed in Saccharomyces cerevisiae, cells grow with a filamentous morphology that we exploited to gain insights into how C. albicans regulates hyphal growth. In S. cerevisiae, INT1-induced filamentous growth was affected by a small subset of actin mutations and a limited set of actin-interacting proteins including Sla2p, anS. cerevisiae protein with similarity in its C terminus to mouse talin. Interestingly, while SLA2 was required forINT1-induced filamentous growth, it was not required for polarized growth in response to several other conditions, suggesting that Sla2p is not required for polarized growth per se. The morphogenesis checkpoint, mediated by Swe1p, contributes toINT1-induced filamentous growth; however, epistasis analysis suggests that Sla2p and Swe1p contribute toINT1-induced filamentous growth through independent pathways. The C. albicans SLA2 homolog (CaSLA2) complements S. cerevisiae sla2Δ mutants for growth at 37°C and INT1-induced filamentous growth. Furthermore, in a C. albicans Casla2/Casla2 strain, hyphal growth did not occur in response to either nutrient deprivation or to potent stimuli, such as mammalian serum. Thus, through analysis ofINT1-induced filamentous growth in S. cerevisiae, we have identified a C. albicans gene,SLA2, that is required for hyphal growth in C. albicans.
机译：白色念珠菌INT1 基因对于菌丝形态发生，粘附和毒力非常重要（C. Gale，C。Bendel，M。McClellan，M。Hauser，JM Becker，J。Berman和M. Hostetter ，科学279：1355-1358，1998年）。在酵母和菌丝形态之间切换的能力是这种真菌病原体中的重要毒力因子。当 Saccharomyces cerevisiae 中表达 INT1 时，细胞会以丝状形态生长，我们利用这些形态来了解 C的方式。白色念珠调节菌丝的生长。在 S中。酿酒酵母， INT1 诱导的丝状生长受到肌动蛋白突变的一小部分和有限的一组肌动蛋白相互作用蛋白（包括Sla2p和 S）的影响。 C蛋白与小鼠塔林蛋白相似。有趣的是，虽然 INT1 诱导的丝状生长需要 SLA2 ，但响应其他几种条件，极化生长并不需要它，这表明极化生长不需要Sla2p。本身。 Swe1p介导的形态发生检查点有助于 INT1 诱导的丝状生长。然而，上位性分析表明Sla2p和Swe1p通过独立的途径促进 INT1 诱导的丝状生长。 C。白色念珠菌SLA2 同源物（ CaSLA2 ）补充了 S。酿酒酵母sla2Δ突变体，可在37°C下生长并 INT1 诱导丝状生长。此外，在 C中。在白色念珠菌Casla2 / Casla2 菌株中，菌丝生长并未因营养缺乏或强刺激（如哺乳动物血清）而发生。因此，通过分析 INT1 诱导的 S中的丝状生长。啤酒，我们确定了 C。 基因， SLA2 ，是 C菌丝生长所必需的。白色的。

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