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首页> 外文期刊>Molecular and Cellular Biology >Purified cofactors and histone H1 mediate transcriptional regulation by CTF/NF-I.
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Purified cofactors and histone H1 mediate transcriptional regulation by CTF/NF-I.

机译：纯化的辅因子和组蛋白H1通过CTF / NF-I介导转录调控。

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摘要

The initiation of RNA polymerase II transcription is controlled by DNA sequence-specific activator proteins, in combination with cofactor polypeptides whose function is poorly understood. Transcriptional cofactors of the CTF-1 activator were purified on the basis of their affinity for the regulatory protein. These purified cofactors were found to be required for CTF-1-regulated transcription, and they counteracted squelching by an excess of activator in in vitro reconstitution experiments. Interestingly, the cofactors possessed an inhibitory activity for basal transcription, which was relieved by the further addition of the activator. Histone H1 also contributes to the regulation of transcription by CTF-1, whereby the activator prevents repression of the basal transcription machinery by the histone. However, histone H1 could not replace the cofactors for CTF-1-regulated transcription, indicating that they possess distinct transcriptional properties. Furthermore, the purified cofactors were found to be required, together with the activator, in order to antagonize the histone-mediated repression of transcription. These results suggest that CTF-1 and its cofactors function by regulating the assembly of the basal transcription machinery onto the promoter when the latter is in competition with DNA-binding inhibitory proteins such as histone H1.
机译：RNA聚合酶II转录的启动受DNA序列特异性激活蛋白与功能知之甚少的辅因子多肽的控制。根据其对调节蛋白的亲和力，可以纯化CTF-1激活子的转录辅助因子。发现这些纯化的辅因子是CTF-1调控转录所必需的，并且它们在体外重构实验中通过过量的活化剂抵消了抑制。有趣的是，辅因子具有对基础转录的抑制活性，通过进一步添加活化剂可以解除这种抑制活性。组蛋白H1还有助于通过CTF-1调节转录，从而使活化剂防止组蛋白抑制基础转录机制。但是，组蛋白H1不能替代CTF-1调控转录的辅因子，表明它们具有独特的转录特性。此外，发现与激活剂一起需要纯化的辅因子，以便拮抗组蛋白介导的转录抑制。这些结果表明，当启动子与DNA结合抑制蛋白（例如组蛋白H1）竞争时，CTF-1及其辅因子通过调节基础转录机制在启动子上的组装发挥功能。

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