Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells.

T Nakashima; T Sekiguchi; A Kuraoka; K Fukushima; Y Shibata; S Komiyama; T Nishimoto

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Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells.

机译：编码新蛋白DAD1的人cDNA的分子克隆，该蛋白的缺陷导致仓鼠BHK21细胞凋亡。

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The tsBN7 cell line, one of the mutant lines temperature sensitive for growth which have been isolated from the BHK21 cell line, was found to die by apoptosis following a shift to the nonpermissive temperature. The induced apoptosis was inhibited by a protein synthesis inhibitor, cycloheximide, but not by the bcl-2-encoded protein. By DNA-mediated gene transfer, we cloned a cDNA that complements the tsBN7 mutation. It encodes a novel hydrophobic protein, designated DAD1, which is well conserved (100% identical amino acids between humans and hamsters). By comparing the base sequences of the parental BHK21 and tsBN7 DAD1 cDNAs, we found that the DAD1-encoding gene is mutated in tsBN7 cells. The DAD1 protein disappeared in tsBN7 cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggers apoptosis.

机译：已发现tsBN7细胞系是对温度敏感的突变株之一，已从BHK21细胞株中分离出来，在转移到非容许温度后，它因凋亡而死亡。诱导的细胞凋亡被蛋白质合成抑制剂环己酰亚胺抑制，但不被bcl-2编码的蛋白质抑制。通过DNA介导的基因转移，我们克隆了一个与tsBN7突变互补的cDNA。它编码一种新的疏水蛋白，命名为DAD1，它非常保守（人和仓鼠之间100％相同的氨基酸）。通过比较亲代BHK21和tsBN7 DAD1 cDNA的碱基序列，我们发现DAD1编码基因在tsBN7细胞中发生了突变。转变为非许可温度后，tsBN7细胞中的DAD1蛋白消失了，这表明DAD1蛋白的丢失会触发细胞凋亡。

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《Molecular and Cellular Biology》 |1993年第10期|共8页
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T Nakashima; T Sekiguchi; A Kuraoka; K Fukushima; Y Shibata; S Komiyama; T Nishimoto;
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中图分类细胞生物学;
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1. cDNA cloning of a defender against apoptotic cell death 1 (DAD1) homologue, responsive to external temperature stimulus from the spider, araneus ventricosus [J] . Kwang Sik Lee, Eun Hwa Chung, Ji Hee Han, Comparative biochemistry and physiology, Part B. Biochemistry & molecular biology . 2003,第1期

机译：对抗凋亡细胞死亡1（DAD1）同源物的防御者的cDNA克隆，其响应蜘蛛，araneus ventricosus的外部温度刺激
2. DNA sequence analysis of NKG2, a family of related cDNA clones encoding type II integral membrane proteins on human natural killer cells. [J] . J P Houchins, T Yabe, C McSherry, The Journal of Experomental Medicine . 1991,第4期

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5. Molecular cloning, localization and functional studies of Rab18, a low molecular weight GTP-binding protein from mouse pituitary cells. [D] . Yu, Helen. 1996

机译：Rab18的分子克隆，定位和功能研究，一种来自小鼠垂体细胞的低分子量GTP结合蛋白。
6. Molecular cloning of a human cDNA encoding a novel protein DAD1 whose defect causes apoptotic cell death in hamster BHK21 cells. [O] . T Nakashima, T Sekiguchi, A Kuraoka, 1993

机译：编码新蛋白DAD1的人类cDNA的分子克隆该蛋白的缺陷导致仓鼠BHK21细胞凋亡。
7. Molecular Cloning of cDNA Encoding a Human Heat-shock Protein Whose Expression is Induced by Adenovirus Type 12 E1A in HeLa Cells [O] . Masaaki Yamazaki, Hiroyuki Tashiro, Kazushige Yokoyama, 1990

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机译：溶酶体膜糖蛋白mLamp-1的cDNa克隆的分离和测序：与携带分化抗原的蛋白质的序列相似性

Molecular cloning of a human cDNA encoding a novel protein, DAD1, whose defect causes apoptotic cell death in hamster BHK21 cells.

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