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首页> 外文期刊>Molecular and Cellular Biology >RNA B is the major nucleolar trimethylguanosine-capped small nuclear RNA associated with fibrillarin and pre-rRNAs in Trypanosoma brucei.
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RNA B is the major nucleolar trimethylguanosine-capped small nuclear RNA associated with fibrillarin and pre-rRNAs in Trypanosoma brucei.

机译：RNA B是布鲁氏锥虫中与原纤维蛋白和pre-rRNA相关的主要的核仁三甲基鸟苷加帽的小核RNA。

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摘要

RNA B is one of three abundant trimethylguanosine-capped U small nuclear RNAs (snRNAs) of Trypanosoma brucei which is not strongly identified with other U snRNAs by sequence homology. We show here that RNA B is a highly diverged U3 snRNA homolog likely involved in pre-rRNA processing. Sequence identity between RNA B and U3 snRNAs is limited; only two of four boxes of homology conserved between U3 snRNAs are obvious in RNA B. These are the box A homology, specific for U3 snRNAs, and the box C homology, common to nucleolar snRNAs and required for association with the nucleolar protein, fibrillarin. A 35-kDa T. brucei fibrillarin homolog was identified by using an anti-Physarum fibrillarin monoclonal antibody. RNA B and fibrillarin were localized in nucleolar fractions of the nucleus which contained pre-rRNAs and did not contain nucleoplasmic snRNAs. Fibrillarin and RNA B were precipitated by scleroderma patient serum S4, which reacts with fibrillarins from diverse organisms; RNA B was the only trimethylguanosine-capped RNA precipitated. Furthermore, RNA B sedimented with pre-rRNAs in nondenaturing sucrose gradients, similarly to U3 and other nucleolar snRNAs, suggesting that RNA B is hydrogen bonded to rRNA intermediates and might be involved in their processing.
机译：RNA B是布氏锥虫的三个丰富的三甲基鸟苷加帽的U小核RNA（snRNA）之一，通过序列同源性未与其他U snRNA强烈鉴定。我们在这里显示RNA B是高度分化的U3 snRNA同源物，可能参与了rRNA的前处理。 RNA B和U3 snRNA之间的序列同一性受到限制;在RNA B中，U3 snRNA之间保守的四个同源盒中只有两个是明显的。这些分别是U3 snRNA特有的Box A同源性和核仁snRNA共有的，与核仁蛋白原纤维蛋白缔合所需的Box C同源性。通过使用抗假单胞菌原纤维素蛋白单克隆抗体鉴定出35 kDa布鲁氏菌原纤维素蛋白同源物。 RNA B和原纤维蛋白位于细胞核的核仁级分中，该核仁级分包含pre-rRNA，但不包含核质snRNA。硬皮病患者血清S4沉淀纤维蛋白和RNA B，该血清S4与来自多种生物的纤维蛋白反应。 RNA B是唯一沉淀的三甲基鸟苷加帽的RNA。此外，与U3和其他核仁snRNA相似，RNA B以pre-rRNA沉淀在非变性蔗糖梯度中，这表明RNA B是氢键合到rRNA中间体的，可能参与了其加工。

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