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首页> 外文期刊>Molecular and Cellular Biology >Developmental regulation and tissue distribution of the liver transcription factor LFB1 (HNF1) in Xenopus laevis.
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Developmental regulation and tissue distribution of the liver transcription factor LFB1 (HNF1) in Xenopus laevis.

机译：非洲爪蟾肝脏转录因子LFB1（HNF1）的发育调节和组织分布。

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The transcription factor LFB1 (HNF1) was initially identified as a regulator of liver-specific gene expression in mammals. It interacts with the promoter element HP1, which is functionally conserved between mammals and amphibians, suggesting that a homologous factor, XLFB1, also exists in Xenopus laevis. To study the role of LFB1 in early development, we isolated two groups of cDNAs coding for this factor from a Xenopus liver cDNA library by using a rat LFB1 cDNA probe. A comparison of the primary structures of the Xenopus and mammalian proteins shows that the myosin-like dimerization helix, the POU-A-related domain, the homeo-domain-related region, and the serine/threonine-rich activation domain are conserved between X. laevis and mammals, suggesting that all these features typical for LFB1 are essential for function. Using monoclonal antibodies, we demonstrate that XLFB1 is present not only in the liver but also in the stomach, intestine, colon, and kidney. In an analysis of the expression of XLFB1 in the developing Xenopus embryo, XLFB1 transcripts appear at the gastrula stage. The XLFB1 protein can be identified in regions of the embryo in which the liver diverticulum, stomach, gut, and pronephros are localized. The early appearance of XLFB1 expression during embryogenesis suggests that the tissue-specific transcription factor XLFB1 is involved in the determination and/or differentiation of specific cell types during organogenesis.
机译：转录因子LFB1（HNF1）最初被确定为哺乳动物中肝特异性基因表达的调节剂。它与启动子元件HP1相互作用，该启动子元件在哺乳动物和两栖动物之间在功能上是保守的，这表明非洲爪蟾中也存在同源因子XLFB1。为了研究LFB1在早期发育中的作用，我们使用大鼠LFB1 cDNA探针从非洲爪蟾肝脏cDNA库中分离出两组编码该因子的cDNA。爪蟾和哺乳动物蛋白的一级结构的比较显示，X之间保守了肌球蛋白样二聚螺旋，POU-A相关结构域，同源异型结构域相关区域以及富含丝氨酸/苏氨酸的激活结构域。 Laevis和哺乳动物，这表明LFB1的所有这些典型特征对于功能都是必不可少的。使用单克隆抗体，我们证明XLFB1不仅存在于肝中，而且还存在于胃，肠，结肠和肾脏中。在对发育中的非洲爪蟾胚胎中XLFB1表达的分析中，XLFB1转录本出现在胃小肠阶段。 XLFB1蛋白可以在肝脏憩室，胃，肠和前肾位于其中的胚胎区域中鉴定。胚胎发生过程中XLFB1表达的早期出现表明组织特异性转录因子XLFB1参与器官发生过程中特定细胞类型的确定和/或分化。

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