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首页> 外文期刊>Molecular and Cellular Biology >The Saccharomyces cerevisiae Hap5p homolog from fission yeast reveals two conserved domains that are essential for assembly of heterotetrameric CCAAT-binding factor.
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The Saccharomyces cerevisiae Hap5p homolog from fission yeast reveals two conserved domains that are essential for assembly of heterotetrameric CCAAT-binding factor.

机译：来自裂变酵母的酿酒酵母Hap5p同源物揭示了两个保守结构域，这对于异源四聚体CCAAT结合因子的组装至关重要。

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摘要

The CCAAT-binding factor is an evolutionarily conserved heteromeric transcription factor that binds to CCAAT box-containing upstream activation sites within the promoters of numerous eukaryotic genes. The CCAAT-binding factor from Saccharomyces cerevisiae is a heterotetramer that contains the subunits Hap2p, Hap3p, Hap4p, and Hap5p and that functions in the activation of genes involved in respiratory metabolism. Here we describe the isolation of the cDNA encoding the Schizosaccharomyces pombe homolog of Hap5p, designated php5+. We have shown that Php5p is a subunit of the CCAAT-binding factor in fission yeast and is required for transcription of the S. pombe cyc1+ gene. Analysis of the evolutionarily conserved regions of Hap5p, Php5p, and the mammalian homolog CBF-C revealed two essential domains within Hap5p that are required for DNA binding and transcriptional activation. One is an 87-amino-acid core domain that is conserved among Hap5p, Php5p, and CBF-C and that is required for the assembly of the Hap2p-Hap3p-Hap5p heterotrimer both in vitro and in vivo. A second domain that is essential for the recruitment of Hap4p into the CCAAT-binding complex was identified in Hap5p and Php5p.
机译：CCAAT结合因子是一种进化保守的异源转录因子，可与许多真核基因启动子内包含CCAAT盒的上游激活位点结合。来自酿酒酵母的CCAAT结合因子是异源四聚体，其包含亚基Hap2p，Hap3p，Hap4p和Hap5p，并在呼吸代谢相关基因的激活中起作用。在这里，我们描述了编码Hap5p（命名为php5 +）的裂殖酵母（Schizosaccharomyces pombe）同源物的cDNA的分离。我们已经显示，Php5p是裂变酵母中CCAAT结合因子的一个亚基，是粟酒裂殖酵母cyc1 +基因转录所必需的。对Hap5p，Php5p和哺乳动物同源CBF-C的进化保守区域的分析显示，Hap5p中有两个必不可少的域，它们是DNA结合和转录激活所必需的。一个是在Hap5p，Php5p和CBF-C之间保守的87个氨基酸的核心域，在体外和体内组装Hap2p-Hap3p-Hap5p异源三聚体都是必需的。在Hap5p和Php5p中鉴定了对Hap4p募集到CCAAT结合复合物中至关重要的第二个域。

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