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首页> 外文期刊>Molecular and Cellular Biology >DDP1, a Heterochromatin-Associated Multi-KH-Domain Protein of Drosophila melanogaster, Interacts Specifically with Centromeric Satellite DNA Sequences
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DDP1, a Heterochromatin-Associated Multi-KH-Domain Protein of Drosophila melanogaster, Interacts Specifically with Centromeric Satellite DNA Sequences

机译：DDP1，果蝇果蝇的杂染色质相关的多KH域蛋白，特别与着丝粒卫星DNA序列相互作用。

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摘要

DDP1 is a single-stranded nucleic acid binding protein ofDrosophila melanogaster that associates with pericentric heterochromatin. DDP1 contains 15 consecutive KH domains and is homologous to the highly conserved vigilin proteins that, inSaccharomyces cerevisiae, are involved in the control of cell ploidy. DDP1 was identified and purified on the basis of its binding to the pyrimidine-rich C strand of the centromericDrosophila dodeca-satellite. Here, the interaction of DDP1 with the dodeca-satellite C strand was analyzed in detail. This interaction is sequence specific. In particular, a guanine residue which is highly conserved in natural dodeca-satellite sequences was found to be essential for the efficient binding of DDP1. DDP1 binding was also found to be strongly influenced by the length and extent of secondary structure of the DNA substrate. Efficient DDP1 binding required a minimal length of about 75 to 100 nucleotides and was facilitated by the lack of secondary structure of the substrate. DDP1 also showed a significant affinity for the unstructured pyrimidine-rich strand of the most abundant centromeric Drosophila AAGAG satellite. The stoichiometry of the complexes formed with the dodeca-satellite C strand suggests that, in DDP1, the 15 consecutive KH domains are organized such that they define two nucleic acid binding surfaces. These results are discussed in the context of the possible contribution of DDP1 to heterochromatin organization and function.
机译：DDP1是果蝇果蝇的单链核酸结合蛋白，与周围异染色质相关。 DDP1包含15个连续的KH结构域，与酿酒酵母中高度保守的守夜蛋白蛋白同源，后者参与细胞倍性的控制。 DDP1是根据其与着丝粒果蝇十二指肠卫星的富嘧啶C链的结合进行鉴定和纯化的。在这里，详细分析了DDP1与十二碳卫星C链的相互作用。该相互作用是序列特异性的。特别地，发现在天然十二-卫星序列中高度保守的鸟嘌呤残基对于DDP1的有效结合是必不可少的。还发现DDP1结合受DNA底物二级结构的长度和程度的强烈影响。有效的DDP1结合需要约75至100个核苷酸的最小长度，并且由于缺乏底物的二级结构而得以促进。 DDP1对最丰富的着丝粒果蝇AAGAG卫星的非结构化富含嘧啶的链也显示出显着的亲和力。与十二烷基卫星C链形成的复合物的化学计量表明，在DDP1中，有15个连续的KH结构域被组织为使得它们定义了两个核酸结合表面。这些结果是在DDP1对异染色质组织和功能的可能贡献的背景下讨论的。

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