Requirement of Rrm3 Helicase for Repair of Spontaneous DNA Lesions in Cells Lacking Srs2 or Sgs1 Helicase

Kristina H. Schmidt; Richard D. Kolodner

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Requirement of Rrm3 Helicase for Repair of Spontaneous DNA Lesions in Cells Lacking Srs2 or Sgs1 Helicase

机译：Rrm3解旋酶修复缺少Srs2或Sgs1解旋酶的细胞中自发性DNA损伤的要求

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The Rrm3 DNA helicase of Saccharomyces cerevisiae interacts with proliferating cell nuclear antigen and is required for replication fork progression through ribosomal DNA repeats and subtelomeric and telomeric DNA. Here, we show that rrm3 srs2 and rrm3 sgs1 mutants, in which two different DNA helicases have been inactivated, exhibit a severe growth defect and undergo frequent cell death. Cells lacking Rrm3 and Srs2 arrest in the G₂/M phase of the cell cycle with 2N DNA content and frequently contain only a single nucleus. The phenotypes of rrm3 srs2 and rrm3 sgs1 mutants were suppressed by disrupting early steps of homologous recombination. These observations identify Rrm3 as a new member of a network of pathways, involving Sgs1 and Srs2 helicases and Mus81 endonuclease, suggested to act during repair of stalled replication forks.

机译：酿酒酵母的Rrm3 DNA解旋酶与增殖细胞核抗原相互作用，并且是通过核糖体DNA重复序列，亚端粒和端粒DNA复制叉进行的过程所必需的。在这里，我们显示了 rrm3 srs2 和 rrm3 sgs1 突变体，其中两种不同的DNA解旋酶已被灭活，它们表现出严重的生长缺陷并经常发生细胞死亡。缺乏Rrm3和Srs2的细胞停滞在细胞周期的G _{2 / M期，DNA含量为2N，通常仅包含一个核。 rrm3 srs2 和 rrm3 sgs1 突变体的表型通过破坏同源重组的早期步骤而受到抑制。这些观察结果表明Rrm3是通路网络的新成员，涉及Sgs1和Srs2解旋酶和Mus81内切核酸酶，建议在停滞的复制叉修复过程中发挥作用。} 展开▼

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《Molecular and Cellular Biology》 |2004年第8期|共14页

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Kristina H. Schmidt; Richard D. Kolodner;
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中图分类细胞生物学;

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1. Requirement of Rrm3 Helicase for Repair of Spontaneous DNA Lesions in Cells Lacking Srs2 or Sgs1 Helicase [J] . Kristina H. Schmidt, Richard D. Kolodner Molecular and Cellular Biology . 2004,第8期

机译：Rrm3解旋酶修复缺少Srs2或Sgs1解旋酶的细胞中自发性DNA损伤的要求

2. Srs2 and Sgs1 DNA Helicases Associate with Mre11 in Different Subcomplexes following Checkpoint Activation and CDK1-Mediated Srs2 Phosphorylation [J] . Irene Chiolo, Walter Carotenuto, Giulio Maffioletti, Molecular and Cellular Biology . 2005,第13期

机译：SRS2和SGS1 DNA螺旋酶在检查点激活和CDK1介导的SRS2磷酸化后的不同子拷贝中缔合的MRE11

3. Heteroduplex DNA Position Defines the Roles of the Sgs1, Srs2, and Mph1 Helicases in Promoting Distinct Recombination Outcomes [J] . Katrina Mitchel, Kevin Lehner, Sue Jinks-Robertson PLoS Genetics . 2013,第3期

机译：异源双链DNA位置定义了Sgs1，Srs2和Mph1解旋酶在促进不同的重组结果中的作用。

4. Isolation and Identification of Helicase from Anoxybacillus sp for DNA amplification [C] . S.Yamuna, I.Asma, B.Venugopal International Conference on Biotechnology and Food Science . 2012

机译：从Anoxybacillus sp进行螺旋酶的分离和鉴定DNA扩增

5. The BLM helicase is involved in the repair of DNA lesions induced by diverse genotoxins. [D] . Behbehani, Gregory K. 2007

机译：BLM解旋酶参与多种遗传毒素诱导的DNA损伤的修复。

6. Requirement of Rrm3 Helicase for Repair of Spontaneous DNA Lesions in Cells Lacking Srs2 or Sgs1 Helicase [O] . Kristina H. Schmidt, Richard D. Kolodner 2001

机译：Rrm3解旋酶修复缺少Srs2或Sgs1解旋酶的细胞中自发性DNA损伤的要求

7. Requirement of Rrm3 Helicase for Repair of Spontaneous DNA Lesions in Cells Lacking Srs2 or Sgs1 Helicase [O] . Schmidt, Kristina H., Kolodner, Richard D. 2004

机译：Rrm3解旋酶修复缺少Srs2或Sgs1解旋酶的细胞中自发性DNA损伤的要求

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2. RecQ酵母解旋酶：DNA修复，基因组稳定性和衰老的线索 [J] . 李星慧 ,孙亮 ,张毓洪 . 中国老年保健医学 . 2015,第005期

3. UvrD解旋酶通过牵动RNA聚合酶反向运动促进DNA修复 [J] . 陈曦 . 生理科学进展 . 2014,第003期

4. 人类解旋酶样转录因子介导辐射凋亡细胞DNA修复蛋白的降解 [J] . 朱应葆 ,韩云 ,孙艳 . 中华放射医学与防护杂志 . 2010,第005期

5. DnaB解旋酶与DnaI装载蛋白结构相互作用的研究进展 [J] . 陈炫志1 . 当代化工研究 . 2019,第001期

6. 染色质解旋酶/ATP酶的DNA结合蛋白1样基因致瘤机制研究进展 [C] . FU Dian ,傅点 ,WANG Ling . 2015年北京放射肿瘤年会 . 2016

7. RecQ解旋酶抗体检测白血病和乳腺癌肿瘤干细胞中解旋酶表达的初步研究 [A] . 廉芳 . 2012

1. 噬菌体TSP4 DNA解旋酶和编码这种解旋酶的多核苷酸 [P] . 中国专利： CN102304500B . 2012.08.29

2. 噬菌体TSP4 DNA解旋酶和编码这种解旋酶的多核苷酸 [P] . 中国专利： CN102304500A . 2012-01-04

3. THREE-DIMENSIONAL STRUCTURE OF A DNAB-FAMILY REPLICATIVE HELICASE (G40P), USES THEREOF, AND METHODS FOR DEVELOPING ANTI-BACTERIAL PATHOGENS BY INHIBITING DNAB HELICASES AND THE INTERACTIONS OF DNAB HELICASE WITH PRIMASE [P] . 外国专利： US2009215075A1 . 2009-08-27

机译：DNAB家族复制解旋酶（G40P）的三维结构及其用途，以及通过抑制DNAB解旋酶来发展抗细菌病原菌的方法以及DNAB解旋酶与本体酶的相互作用

4. DNA AMPLIFICATION METHOD USING DEAD-box RNA HELICASE DERIVED FROM THERMOPHILIC ARCHAEA OR VARIANT THEREOF, AND DEAD-box RNA HELICASE DERIVED FROM THERMOPHILIC ARCHAEA AND VARIANT THEREOF TO BE USED FOR DNA AMPLIFICATION METHOD [P] . 外国专利： WO2016013620A1 . 2016-01-28

机译：DNA扩增方法，使用衍生自嗜热古生菌或其变种的死箱RNA解旋酶和衍生自dead-box RNA解旋酶及其变种的DNA，用于DNA扩增方法

5. CELL CYCLE REPORTING CELL LINE COMPRISING PHOSPHORYLATED SUBCELLULAR LOCALIZATION DOMAIN (PSLD) OF HUMAN DNA HELICASE B (HDBD) [P] . 外国专利： CA2574540A1 . 2006-01-26

机译：包含人类DNA解旋酶B（HDBD）的磷酸化亚细胞定位域（PSLD）的细胞周期报告细胞系

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Requirement of Rrm3 Helicase for Repair of Spontaneous DNA Lesions in Cells Lacking Srs2 or Sgs1 Helicase

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