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首页> 外文期刊>Molecular and Cellular Biology >Sox7 Plays Crucial Roles in Parietal Endoderm Differentiation in F9 Embryonal Carcinoma Cells through Regulating Gata-4 and Gata-6 Expression
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Sox7 Plays Crucial Roles in Parietal Endoderm Differentiation in F9 Embryonal Carcinoma Cells through Regulating Gata-4 and Gata-6 Expression

机译：Sox7通过调节Gata-4和Gata-6的表达在F9胚胎癌细胞顶叶内胚层分化中起关键作用。

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During early rodent development, the parietal endoderm appears from an inner cell mass and produces large amounts of basement membrane components, such as laminin-1 and collagen IV. To elucidate the regulatory network for gene expression during these procedures, we constructed a series of short interfering RNA expression vectors targeted to various transcription factors, transfected them into F9 embryonal carcinoma cells, and evaluated the effects of the gene silencing on the induction of parietal endoderm differentiation and basement membrane component production by treating F9 cells with all trans-retinoic acid and dibutyryl cyclic AMP. Among the transcription factors tested, silencing of Sox7 or combined silencing of Gata-4 and Gata-6 resulted in suppression of cell shape changes and laminin-1 production, which are the hallmarks of parietal endoderm differentiation. In cells silenced for Sox7, induction of Gata-4 and Gata-6 by retinoic acid and cyclic AMP treatment was inhibited, while induction of Sox7 was not affected in cells silenced for Gata-4 and Gata-6, indicating that Sox7 is an upstream regulatory factor for these Gata factors. Nevertheless, silencing of Sox7 did not totally cancel the action of retinoic acid, since upregulation of coup-tf2, keratin 19, and retinoic acid receptor β2 was not abolished in Sox7-silenced F9 cells. Although overexpression of Sox7 alone was insufficient to induce parietal endoderm differentiation, overexpression of Gata-4 or Gata-6 in Sox7-silenced F9 cells restored the differentiation into parietal endoderm. Sox7 is therefore required for the induction of Gata-4 and Gata-6, and the interplay among these transcription factors plays a crucial role in parietal endoderm differentiation.
机译：在早期啮齿动物的发育过程中，顶叶内胚层从内部细胞团中出现，并产生大量的基底膜成分，例如层粘连蛋白-1和胶原蛋白IV。为了阐明这些程序中基因表达的调控网络，我们构建了一系列针对各种转录因子的短干扰RNA表达载体，将其转染到F9胚胎癌细胞中，并评估了基因沉默对顶叶内胚层诱导的影响用所有反式-视黄酸和二丁酰基环AMP处理F9细胞可分化和分化基底膜成分。在测试的转录因子中，Sox7沉默或Gata-4和Gata-6的组合沉默导致细胞形状变化和层粘连蛋白1产生的抑制，这是顶叶内胚层分化的标志。在针对Sox7沉默的细胞中，视黄酸和循环AMP处理对Gata-4和Gata-6的诱导被抑制，而对于针对Gata-4和Gata-6沉默的细胞，Sox7的诱导不受影响，这表明Sox7是上游这些Gata因子的调节因子。但是，由于 coup-tf2 ，角蛋白19 和视黄酸受体的上调，Sox7的沉默并不能完全消除视黄酸的作用。在Sox7沉默的F9细胞中，β 2 没有消失。尽管仅Sox7的过表达不足以诱导顶内胚层分化，但Sox7沉默的F9细胞中Gata-4或Gata-6的过表达恢复了分化为顶内胚层。因此，Sox7是诱导Gata-4和Gata-6所必需的，这些转录因子之间的相互作用在顶叶内胚层分化中起着至关重要的作用。

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