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首页> 外文期刊>Molecular and Cellular Biology >Selective Requirement for SAGA in Hog1-Mediated Gene Expression Depending on the Severity of the External Osmostress Conditions
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Selective Requirement for SAGA in Hog1-Mediated Gene Expression Depending on the Severity of the External Osmostress Conditions

机译：Hog1介导的基因表达中SAGA的选择性要求取决于外部渗透压条件的严重性

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Regulation of gene expression by the Hog1 stress-activated protein kinase is essential for proper cell adaptation to osmostress. Hog1 coordinates an extensive transcriptional program through the modulation of transcription. To identify systematically novel components of the transcriptional machinery required for osmostress-mediated gene expression, we performed an exhaustive genome-wide genetic screening, searching for mutations that render cells osmosensitive at high osmolarity and that are associated with reduced expression of osmoresponsive genes. The SAGA and Mediator complexes were identified as putative novel regulators of osmostress-mediated transcription. Interestingly, whereas Mediator is essential for osmostress gene expression, the requirement for SAGA is different depending on the strength of the extracellular osmotic conditions. At mild osmolarity, SAGA mutants show only very slight defects on RNA polymerase II (Pol II) recruitment and gene expression, whereas at severe osmotic conditions, SAGA mutants show completely impaired RNA Pol II recruitment and transcription of osmoresponsive genes. Thus, our results define an essential role for Mediator in osmostress gene expression and a selective role for SAGA under severe osmostress. Our results indicate that the requirement for a transcriptional complex to regulate a promoter might be determined by the strength of the stimuli perceived by the cell through the regulation of interactions between transcriptional complexes.
机译：Hog1应力激活的蛋白激酶对基因表达的调节对于适当的细胞适应渗透压至关重要。 Hog1通过转录调节来协调广泛的转录程序。为了确定渗透压介导的基因表达所需的转录机制的系统新颖成分，我们进行了详尽的全基因组遗传筛选，寻找使细胞在高渗透压下具有渗透压敏感性并与渗透压反应性基因表达降低相关的突变。 SAGA和介体复合物被认为是由渗透压介导的转录的新型调节剂。有趣的是，尽管介体对于渗透压基因表达是必不可少的，但SAGA的要求因细胞外渗透条件的强度而异。在适度的渗透压下，SAGA突变体在RNA聚合酶II（Pol II）募集和基因表达上仅表现出非常轻微的缺陷，而在严重的渗透压条件下，SAGA突变体则显示了渗透应答基因的RNA Pol II募集和转录完全受损。因此，我们的结果确定了介导剂在渗透压基因表达中的重要作用，以及在严重渗透压下SAGA的选择性作用。我们的结果表明，转录复合物调控启动子的要求可能由细胞通过转录复合物之间相互作用的调控所感知的刺激强度来决定。

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