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首页> 外文期刊>Molecular and Cellular Biology >Cross-binding of factors to functionally different promoter elements in c-fos and skeletal actin genes.
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Cross-binding of factors to functionally different promoter elements in c-fos and skeletal actin genes.

机译：因子与c-fos和骨骼肌肌动蛋白基因中功能不同的启动子元件的交叉结合。

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A conserved 28-base-pair element in the skeletal actin promoter was sufficient to activate muscle-specific expression when placed upstream of a TATA element. This muscle regulatory element (MRE) is similar in structure to the serum response element (SRE), which is present in the promoters of the c-fos proto-oncogene and the nonmuscle actin genes. The SRE can function as a constitutive promoter element. Though the MRE and SRE differed in their tissue-specific expression properties, both elements bound to the same protein factors in vitro. These proteins are the serum response factor (SRF) and the muscle actin promoter factors 1 and 2 (MAPF1 and MAPF2). The SRF and MAPF proteins were resolved by chromatographic procedures, and they differed in their relative affinities for each element. The factors were further distinguished by their distinct, but overlapping, methylation interference footprint patterns on each element. These data indicate that the differences in tissue-specific expression may be due to a complex interaction of protein factors with these sequences.
机译：当放置在TATA元件上游时，骨骼肌动蛋白启动子中保守的28个碱基对元件足以激活肌肉特异性表达。此肌肉调节元件（MRE）在结构上与血清反应元件（SRE）类似，后者存在于c-fos原癌基因和非肌动蛋白基因的启动子中。 SRE可以用作组成型启动子元件。尽管MRE和SRE在组织特异性表达特性上有所不同，但两种元素在体外均与相同的蛋白质因子结合。这些蛋白质是血清反应因子（SRF）和肌肉肌动蛋白启动子因子1和2（MAPF1和MAPF2）。 SRF和MAPF蛋白通过色谱方法分离，每种元素的相对亲和力不同。这些因素通过在每个元素上的独特但重叠的甲基化干扰足迹模式进一步区分。这些数据表明组织特异性表达的差异可能是由于蛋白质因子与这些序列的复杂相互作用所致。

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