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首页> 外文期刊>Molecular and Cellular Biology >Phosphatidylinositol kinase activity associates with viral p60src protein.
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Phosphatidylinositol kinase activity associates with viral p60src protein.

机译：磷脂酰肌醇激酶活性与病毒p60src蛋白有关。

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摘要

Immunoprecipitates of p60v-src proteins from chicken embryo fibroblasts infected with Rous sarcoma virus were assayed for phosphatidylinositol (PI) kinase activity in the absence of detergents. The product of the PI kinase reaction, phosphatidylinositol monophosphate (PIP), migrated slightly slower than did the authentic phosphatidylinositol-4-monophosphate marker in thin-layer chromatography and was indistinguishable from phosphatidylinositol-3-monophosphate produced by PI kinase type I. Furthermore, the deacylated product comigrated with glycerophosphoinositol-3-phosphate in high-performance liquid chromatography. Both sucrose gradient fractionation and the heat stability of PI kinase activity from cells infected with temperature-sensitive mutants suggest that the PI kinase activity is not intrinsic to p60v-src but is a property of another molecule complexed with p60v-src. All transforming variants of p60src were associated with PI kinase activity, whereas this enzyme activity was hardly detectable in immunoprecipitates from cells infected with nontransforming viruses encoding p60c-src or an enzymatically inactive variant. However, PI kinase activity was found in p60src immunoprecipitates from cells infected with nonmyristylated, nontransforming mutants as well as temperature-sensitive mutants at the nonpermissive temperature, which indicated that simple association of PI kinase activity with p60src is not sufficient for cell transformation.
机译：在没有去污剂的情况下，测定了感染了劳斯肉瘤病毒的鸡胚成纤维细胞中p60v-src蛋白的免疫沉淀物的磷脂酰肌醇（PI）激酶活性。 PI激酶反应的产物磷脂酰肌醇一磷酸（PIP）在薄层色谱中的迁移速度比真正的磷脂酰肌醇-4-单磷酸标记物稍慢，并且与I型PI激酶产生的磷脂酰肌醇-3-单磷酸没有区别。在高效液相色谱中将脱酰产物与甘油磷酸肌醇-3-磷酸酯混合。来自被温度敏感突变体感染的细胞的蔗糖梯度分级分离和PI激酶活性的热稳定性都表明PI激酶活性不是p60v-src固有的，而是另一种与p60v-src复合的分子的特性。 p60src的所有转化变体均与PI激酶活性相关，而在编码p60c-src或非酶活性变体的非转化病毒感染的细胞的免疫沉淀物中很难检测到该酶活性。然而，在非许可温度下，感染了非肉豆蔻基化，非转化突变体和温度敏感突变体的细胞在p60src免疫沉淀物中发现了PI激酶活性，这表明PI激酶活性与p60src的简单关联不足以进行细胞转化。

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