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首页> 外文期刊>Molecular and Cellular Biology >The major histocompatibility complex class II promoter-binding protein RFX (NF-X) is a methylated DNA-binding protein.
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The major histocompatibility complex class II promoter-binding protein RFX (NF-X) is a methylated DNA-binding protein.

机译：主要的组织相容性复合物II类启动子结合蛋白RFX（NF-X）是甲基化的DNA结合蛋白。

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A mammalian protein called RFX or NF-X binds to the X box (or X1 box) in the promoters of a number of major histocompatibility (MHC) class II genes. In this study, RFX was shown to have the same DNA-binding specificity as methylated DNA-binding protein (MDBP), and its own cDNA was found to contain a binding site for MDBP in the leader region. MDBP is a ubiquitous mammalian protein that binds to certain DNA sequences preferentially when they are CpG methylated and to other related sequences, like the X box, irrespective of DNA methylation. MDBP from HeLa and Raji cells formed DNA-protein complexes with X-box oligonucleotides that coelectrophoresed with those containing standard MDBP sites. Furthermore, MDBP and X-box oligonucleotides cross-competed for the formation of these DNA-protein complexes. DNA-protein complexes obtained with MDBP sites displayed the same partial supershifting with an antiserum directed to the N terminus of RFX seen for complexes containing an X-box oligonucleotide. Also, the in vitro-transcribed-translated product of a recombinant RFX cDNA bound specifically to MDBP ligands and displayed the DNA methylation-dependent binding of MDBP. RFX therefore contains MDBP activity and thereby also EF-C, EP, and MIF activities that are indistinguishable from MDBP and that bind to methylation-independent sites in the transcriptional enhancers of polyomavirus and hepatitis B virus and to an intron of c-myc.
机译：称为RFX或NF-X的哺乳动物蛋白与许多II类主要组织相容性（MHC）基因的启动子中的X框（或X1框）结合。在这项研究中，RFX被证明具有与甲基化DNA结合蛋白（MDBP）相同的DNA结合特异性，并且发现它自己的cDNA在前导区中含有MDBP的结合位点。 MDBP是一种普遍存在的哺乳动物蛋白，当某些DNA序列被CpG甲基化时，它们优先与某些DNA序列结合，而与其他相关序列（如X盒）结合，而与DNA甲基化无关。来自HeLa和Raji细胞的MDBP与X-box寡核苷酸形成DNA-蛋白质复合物，该寡核苷酸与含有标准MDBP位点的寡核苷酸共电泳。此外，MDBP和X-box寡核苷酸相互竞争以形成这些DNA-蛋白质复合物。用MDBP位点获得的DNA-蛋白质复合物显示出相同的部分超移位，其中抗血清针对包含X-box寡核苷酸的复合物，指向RFX的N端。同样，重组RFX cDNA的体外转录翻译产物与MDBP配体特异性结合，并表现出DNA甲基化依赖性的MDBP结合。因此，RFX包含MDBP活性，因此还包含与MDBP不可区分的EF-C，EP和MIF活性，它们与多瘤病毒和乙型肝炎病毒的转录增强子中的甲基化非依赖性位点以及c-myc内含子结合。

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