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首页> 外文期刊>Molecular and Cellular Biology >MKK1 and MKK2, which encode Saccharomyces cerevisiae mitogen-activated protein kinase-kinase homologs, function in the pathway mediated by protein kinase C.
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MKK1 and MKK2, which encode Saccharomyces cerevisiae mitogen-activated protein kinase-kinase homologs, function in the pathway mediated by protein kinase C.

机译：编码啤酒酵母促细胞分裂素激活的蛋白激酶激酶同源物的MKK1和MKK2在蛋白激酶C介导的途径中起作用。

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The PKC1 gene of Saccharomyces cerevisiae encodes a homolog of mammalian protein kinase C that is required for normal growth and division of yeast cells. We report here the isolation of the yeast MKK1 and MKK2 (for mitogen-activated protein [MAP] kinase-kinase) genes which, when overexpressed, suppress the cell lysis defect of a temperature-sensitive pkc1 mutant. The MKK genes encode protein kinases most similar to the STE7 product of S. cerevisiae, the byr1 product of Schizosaccharomyces pombe, and vertebrate MAP kinase-kinases. Deletion of either MKK gene alone did not cause any apparent phenotypic defects, but deletion of both MKK1 and MKK2 resulted in a temperature-sensitive cell lysis defect that was suppressed by osmotic stabilizers. This phenotypic defect is similar to that associated with deletion of the BCK1 gene, which is thought to function in the pathway mediated by PCK1. The BCK1 gene also encodes a predicted protein kinase. Overexpression of MKK1 suppressed the growth defect caused by deletion of BCK1, whereas an activated allele of BCK1 (BCK1-20) did not suppress the defect of the mkk1 mkk2 double disruption. Furthermore, overexpression of MPK1, which encodes a protein kinase closely related to vertebrate MAP kinases, suppressed the defect of the mkk1 mkk2 double mutant. These results suggest that MKK1 and MKK2 function in a signal transduction pathway involving the protein kinases encoded by PKC1, BCK1, and MPK1. Genetic epistasis experiments indicated that the site of action for MKK1 and MKK2 is between BCK1 and MPK1.
机译：酿酒酵母的PKC1基因编码哺乳动物蛋白激酶C的同源物，这是酵母细胞正常生长和分裂所必需的。我们在这里报告的酵母MKK1和MKK2（促分裂原活化蛋白[MAP]激酶激酶）基因的分离，当过表达时，抑制温度敏感pkc1突变体的细胞裂解缺陷。 MKK基因编码的蛋白激酶与酿酒酵母的STE7产物，粟酒裂殖酵母的BYR1产物和脊椎动物MAP激酶激酶最相似。单独删除任何一个MKK基因都不会引起任何明显的表型缺陷，但是MKK1和MKK2的缺失均会导致温度敏感的细胞裂解缺陷，并被渗透稳定剂抑制。这种表型缺陷类似于与BCK1基因缺失有关的缺陷，后者被认为在PCK1介导的途径中起作用。 BCK1基因还编码一个预测的蛋白激酶。 MKK1的过表达抑制了BCK1缺失引起的生长缺陷，而激活的BCK1等位基因（BCK1-20）却不能抑制mkk1 mkk2双重破坏的缺陷。此外，编码与脊椎动物MAP激酶密切相关的蛋白激酶的MPK1的过表达抑制了mkk1 mkk2双突变体的缺陷。这些结果表明，MKK1和MKK2在涉及PKC1，BCK1和MPK1编码的蛋白激酶的信号转导途径中起作用。遗传上位实验表明，MKK1和MKK2的作用位点在BCK1和MPK1之间。

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