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首页> 外文期刊>Molecular and Cellular Biology >Raf-1 protein kinase is important for progesterone-induced Xenopus oocyte maturation and acts downstream of mos.
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Raf-1 protein kinase is important for progesterone-induced Xenopus oocyte maturation and acts downstream of mos.

机译：Raf-1蛋白激酶对于孕激素诱导的爪蟾卵母细胞成熟很重要，并在mos下游起作用。

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In somatic cells, the Raf-1 serine/threonine protein kinase is activated by several polypeptide growth factors. We investigated the role of Raf-1 in progesterone-induced meiotic maturation of Xenopus laevis oocytes. Raf-1 enzymatic activity and phosphorylation (reflected by a mobility shift on sodium dodecyl sulfate gels) were increased in oocytes following progesterone stimulation. The increase in Raf-1 activity was concurrent with an elevation in the activity of mitogen-activated protein (MAP) kinase. When RNA encoding an oncogenic form of Raf-1 (v-Raf) was injected into immature oocytes, MAP kinase mobility shift, germinal vesicle breakdown, and histone H1 phosphorylation increased markedly. When RNA encoding a dominant-negative version of Raf-1 was injected, progesterone-induced oocyte maturation was blocked. When RNA encoding Xenopus mos (mosxe) was injected into oocytes, Raf-1 and MAP kinase mobility shifts were observed after several hours. Also, when antisense mosxe oligonucleotides were injected into oocytes, progesterone-induced Raf-1 and MAP kinase mobility shifts were blocked. Finally, when antisense mosxe oligonucleotides were coinjected with v-Raf RNA into oocytes, histone H1 kinase activation, germinal vesicle breakdown, and MAP kinase mobility shift occurred. These findings suggest that Raf-1 activity is required for progesterone-induced oocyte maturation and that Raf-1 is downstream of mosxe activity.
机译：在体细胞中，Raf-1丝氨酸/苏氨酸蛋白激酶被几种多肽生长因子激活。我们调查了Raf-1在非洲爪蟾卵母细胞孕酮诱导的减数分裂成熟中的作用。孕酮刺激后，卵母细胞中Raf-1的酶活性和磷酸化（反映在十二烷基硫酸钠凝胶上的迁移率改变）。 Raf-1活性的增加与丝裂原激活蛋白（MAP）激酶活性的增加同时发生。当将编码致癌形式的Raf-1（v-Raf）的RNA注入未成熟的卵母细胞时，MAP激酶迁移率移动，生小泡分解和组蛋白H1磷酸化显着增加。当注射编码Raf-1显性阴性版本的RNA时，孕酮诱导的卵母细胞成熟被阻断。当将编码非洲爪蟾（moseno）的RNA注入卵母细胞后，数小时后观察到Raf-1和MAP激酶迁移率变化。同样，当将反义mosxe寡核苷酸注射到卵母细胞中时，孕激素诱导的Raf-1和MAP激酶迁移率转移被阻断。最后，当反义mosxe寡核苷酸与v-Raf RNA共同注入卵母细胞时，发生组蛋白H1激酶激活，生小泡破坏和MAP激酶迁移率移动。这些发现表明，Raf-1活性是孕激素诱导的卵母细胞成熟所必需的，并且Raf-1在mosxe活性的下游。

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