...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Identification of a cell-type-specific and E2F-independent mechanism for repression of cdc2 transcription.
【24h】

Identification of a cell-type-specific and E2F-independent mechanism for repression of cdc2 transcription.

机译：鉴定一种细胞类型特异性和E2F依赖性机制来抑制cdc2转录。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Human myeloid leukemia cells, such as HL60, U937, and THP1 cells, undergo macrophage differentiation and growth arrest following treatment with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). Surprisingly, we find that growth of a significant percentage of THP1 cells is arrested in the G2 phase of the cell cycle. G2 arrest correlates with cell-specific repression of the gene encoding p34cdc2, a crucial regulator of G2/M progression. Intriguingly, TPA-mediated repression of the cdc2 promoter was independent of the transcription factor E2F, distinguishing this pathway from mechanisms responsible for repression of cdc2 transcription in response to serum starvation. The region of the cdc2 promoter required for repression was located from bp -22 to -2 from the major transcriptional start site. This sequence, which we term the R box, directs the uncoupling of the basal promoter from upstream activators following TPA treatment. Analysis of THP1 nuclear proteins revealed a 55-kDa protein that was induced by TPA and interacted with the cdc2 promoter in an R-box-dependent manner. These observations provide evidence for the existence of cell-type- and promoter-specific pathways for the assembly of stable transcriptional initiation complexes that function to differentially regulate the expression of cell cycle control genes in mammalian cells.
机译：在用佛波醇酯12-O-十四烷酰佛波醇13-乙酸酯（TPA）处理后，人类髓样白血病细胞（例如HL60，U937和THP1细胞）经历巨噬细胞分化和生长停滞。出乎意料的是，我们发现THP1细胞的显着增长被阻止在细胞周期的G2期。 G2逮捕与编码p34cdc2（G2 / M进展的关键调节因子）的基因的细胞特异性抑制有关。有趣的是，TPA介导的对cdc2启动子的阻遏独立于转录因子E2F，从而将该途径与负责响应血清饥饿的cdc2转录阻遏的机制区分开来。阻遏所需的cdc2启动子区域位于主要转录起始位点的bp -22至-2之间。在TPA处理后，我们称此序列为R盒的该序列指导基础启动子与上游激活剂的解偶联。对THP1核蛋白的分析揭示了一个55 kDa的蛋白，该蛋白被TPA诱导并以c-box依赖性方式与cdc2启动子相互作用。这些观察提供了用于稳定转录起始复合物装配的细胞类型和启动子特异性途径的存在的证据，所述功能用于差异调节哺乳动物细胞中细胞周期控制基因的表达。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号