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首页> 外文期刊>Molecular and Cellular Biology >Cell cycle-regulated association of E2F1 and Sp1 is related to their functional interaction.
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Cell cycle-regulated association of E2F1 and Sp1 is related to their functional interaction.

机译：E2F1和Sp1的细胞周期调节关联与它们的功能相互作用有关。

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Because of the large number of growth-regulated genes containing binding sites for the transcription factors Sp1 and E2F and the reported ability of E2F to mediate cell cycle (growth) regulation, we studied interactions between E2F1 and Sp1. In transient transfection assays using Drosophila melanogaster SL2 cells, transfection with both Sp1 and E2F1 expression vectors resulted in greater than 85-fold activation of transcription from a hamster dihydrofolate reductase reporter construct, whereas cotransfection with either the Sp1 or E2F1 expression vector resulted in 30- or <2-fold activation, respectively. Therefore, these transcription factors act synergistically in activation of dihydrofolate reductase transcription. Transient transfection studies demonstrated that E2F1 could superactivate Sp1-dependent transcription in a promoter containing only Sp1 sites and that Sp1 could superactivate transcription of promoters through E2F sites, further demonstrating that these physically associated in Drosophila cells transfected with Sp1 and E2F1 expression vectors and in human cells, with maximal interaction detected in mid- to late G1. Additionally, E2F1 and Sp1 interact in vitro through specific domains of each protein, and the physical interaction and functional synergism appear to require the same regions. Taken together, these data demonstrate that E2F1 and Sp1 both functionally and physically interact; therefore this interaction, Sp1 and E2F1 may regulate transcription of genes containing binding sites for either or both factors.
机译：由于大量的生长调节基因包含转录因子Sp1和E2F的结合位点，并且据报道E2F介导细胞周期（生长）调节的能力，我们研究了E2F1和Sp1之间的相互作用。在使用果蝇SL2细胞进行的瞬时转染测定中，用Sp1和E2F1表达载体进行转染均可导致仓鼠二氢叶酸还原酶报道基因构建体的转录激活超过85倍，而与Sp1或E2F1表达载体共转染可导致30-或<2倍激活。因此，这些转录因子在二氢叶酸还原酶转录的激活中协同作用。瞬时转染研究表明，E2F1可以在仅包含Sp1位点的启动子中超激活Sp1依赖性转录，而Sp1可以通过E2F位点超激活启动子的转录，进一步证明这些在转染了Sp1和E2F1表达载体的果蝇细胞中以及在人类中都存在物理关联细胞，在G1的中晚期检测到最大的相互作用。此外，E2F1和Sp1通过每种蛋白质的特定结构域在体外相互作用，并且物理相互作用和功能协同作用似乎需要相同的区域。这些数据加在一起表明E2F1和Sp1在功能和物理上都相互作用。因此，Sp1和E2F1的这种相互作用可能会调节含有一个或两个因子结合位点的基因的转录。

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