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首页> 外文期刊>Molecular and Cellular Biology >Complex architecture of major histocompatibility complex class II promoters: reiterated motifs and conserved protein-protein interactions.
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Complex architecture of major histocompatibility complex class II promoters: reiterated motifs and conserved protein-protein interactions.

机译：主要组织相容性复合物II类启动子的复合物结构：重申的基序和保守的蛋白质-蛋白质相互作用。

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The S box (also known as at the H, W, or Z box) is the 5'-most element of the conserved upstream sequences in promoters of major histocompatibility complex class II genes. It is important for their B-cell-specific and interferon gamma-inducible expression. In this study, we demonstrate that the S box represents a duplication of the downstream X box. First, RFX, which is composed of the RFX5-p36 heterodimer that binds to the X box, also binds to the S box and its 5'-flanking sequence. Second, NF-Y, which binds to the Y box and increases interactions between RFX and the X box, also increases the binding of RFX to the S box. Third, RFXs bound to S and X boxes interact with each other in a spatially constrained manner. Finally, we confirmed these protein-protein and protein-DNA interactions by expressing a hybrid RFX5-VP16 protein in cells. We conclude that RFX binds to S and X boxes and that complex interactions between RFX and NF-Y direct B-cell-specific and interferon gamma-inducible expression or major histocompatibility complex class II genes.
机译：S盒（也称为H，W或Z盒）是主要组织相容性复杂II类基因启动子中保守上游序列的5'-最末端元素。对于它们的B细胞特异性和γ干扰素诱导表达非常重要。在这项研究中，我们证明了S盒代表下游X盒的重复。首先，RFX由与X盒结合的RFX5-p36异二聚体组成，也与S盒及其5'侧翼序列结合。其次，NF-Y绑定到Y框并增加RFX和X框之间的相互作用，也增加RFX到S框的绑定。第三，绑定到S和X框的RFX以空间受限的方式相互交互。最后，我们通过在细胞中表达杂种RFX5-VP16蛋白来确认这些蛋白-蛋白和蛋白-DNA相互作用。我们得出的结论是，RFX与S和X盒结合，并且RFX和NF-Y之间的复杂相互作用直接指导B细胞特异性和干扰素γ诱导的表达或主要的组织相容性复杂的II类基因。

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