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首页> 外文期刊>Molecular and Cellular Biology >TFIIH functions in regulating transcriptional elongation by RNA polymerase II in Xenopus oocytes.
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TFIIH functions in regulating transcriptional elongation by RNA polymerase II in Xenopus oocytes.

机译：TFIIH在非洲爪蟾卵母细胞中通过RNA聚合酶II调节转录伸长。

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We investigated the role of TFIIH in transcription by RNA polymerase II (pol II) in vivo by microinjection of antibodies against this factor into Xenopus oocytes. Five different antibodies directed against four subunits of TFIIH were tested for effects on transcription of coinjected human immunodeficiency virus type 2 and c-myc templates. Each of these antibodies severely reduced the efficiency of elongation through human immunodeficiency virus type 2 and c-myc terminator elements. In contrast, an anti-TFIIB antibody did not inhibit elongation. Anti-TFIIH antibodies also had a much smaller inhibitory effect on total transcription than did anti-TFIIB or anti-pol II large subunit. Three inhibitors of TFIIH kinase activity, H-7, H-8, and dichlororibofuranosylbenzimidazole (DRB), inhibited elongation similarly to anti-TFIIH antibodies. These results strongly suggest a role for TFIIH in the stimulation of transcriptional elongation in vivo.
机译：我们通过在非洲爪蟾卵母细胞中微注射针对该因子的抗体，研究了TFIIH在RNA聚合酶II（pol II）体内转录中的作用。测试了针对TFIIH四个亚基的五种不同抗体对共注射人2型免疫缺陷病毒和c-myc模板转录的影响。这些抗体中的每一种都通过2型人类免疫缺陷病毒和c-myc终止子元件严重降低了延伸效率。相反，抗TFIIB抗体不抑制延伸。抗-TFIIH抗体对总转录的抑制作用也比抗-TFIIB或抗-pol II大亚基小得多。 TFIIH激酶活性的三种抑制剂H-7，H-8和二氯呋喃核糖基苯并咪唑（DRB）与抗TFIIH抗体相似，可抑制伸长。这些结果强烈暗示了TFIIH在体内刺激转录伸长中的作用。

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