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首页> 外文期刊>Molecular and Cellular Biology >The Oct-1 POU-specific domain can stimulate small nuclear RNA gene transcription by stabilizing the basal transcription complex SNAPc.
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The Oct-1 POU-specific domain can stimulate small nuclear RNA gene transcription by stabilizing the basal transcription complex SNAPc.

机译：Oct-1 POU特异性结构域可以通过稳定基础转录复合体SNAPc刺激小核RNA基因转录。

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摘要

The RNA polymerase II and III human small nuclear RNA promoters have a common basal element, the proximal sequence element, which binds the TATA box-binding protein-containing complex SNAPc. They also contain an enhancer characterized by a highly conserved octamer sequence, which constitutes a binding site for the broadly expressed POU domain transcription factor Oct-1. The POU domain is a bipartite DNA-binding domain consisting of a POU-homeo (POUH) domain and a POU-specific (POUs) domain joined by a flexible linker. Here, we show that the Oct-1 POU domain but not the related Pit-1 POU domain can facilitate the binding of SNAPc to the proximal sequence element, and activate transcription. The effect is probably mediated by protein-protein contacts, and 1 of 30 amino acid differences between the Oct-1 and Pit-1 POUs domains is the key determinant for the differential interaction with SNAPc and the ability to activate transcription. These results show that a function that is the hallmark of activation domains, namely, recruitment of a basal transcription complex resulting in activation of transcription, can be performed by a DNA-binding domain. In this case, subtle changes between activator DNA-binding domains, as subtle as a single amino acid difference, can profoundly affect interaction with the basal transcription machinery.
机译：RNA聚合酶II和III人类小核RNA启动子具有共同的基础元素，即近端序列元素，可与包含TATA盒结合蛋白的复合物SNAPc结合。它们还含有特征为高度保守的八聚体序列的增强子，该八聚体序列构成了广泛表达的POU结构域转录因子Oct-1的结合位点。 POU域是一个双向DNA结合域，由一个POU-homeo（POUH）域和一个POU特定（POUs）域通过一个灵活的连接子连接而成。在这里，我们显示Oct-1 POU结构域而不是相关的Pit-1 POU结构域可以促进SNAPc与近端序列元件的结合，并激活转录。该作用可能是由蛋白质-蛋白质接触介导的，Oct-1和Pit-1 POU结构域之间30个氨基酸的差异之一是与SNAPc差异相互作用和激活转录能力的关键决定因素。这些结果表明，可以通过DNA结合域来执行作为激活域的特征的功能，即募集导致转录激活的基础转录复合物。在这种情况下，激活剂DNA结合结构域之间的细微变化（如单个氨基酸差异一样）可以深刻影响与基础转录机制的相互作用。

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