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首页> 外文期刊>Molecular and Cellular Biology >A novel role for Cdc5p in DNA replication.
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A novel role for Cdc5p in DNA replication.

机译：Cdc5p在DNA复制中的新作用。

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DNA replication initiates from specific chromosomal sites called origins, and in the budding yeast Saccharomyces cerevisiae these sites are occupied by the origin recognition complex (ORC). Dbf4p is proposed to play a role in targeting the G1/S kinase Cdc7p to initiation complexes late in G1. We report that Dbf4p may also recruit Cdc5p to origin complexes. Cdc5p is a member of the Polo family of kinases that is required for the completion of mitosis. Cdc5p and Cdc7p each interact with a distinct domain of Dbf4p. cdc5-1 mutants have a plasmid maintenance defect that can be suppressed by the addition of multiple origins. cdc5-1 orc2-1 double mutants are synthetically lethal. Levels of Cdc5p were found to be cell cycle regulated and peaked in G2/M. These results suggest a role for Cdc5p and possibly Polo-like kinases at origin complexes.
机译：DNA复制从称为起源的特定染色体位点开始，在芽生的酿酒酵母中，这些位点被起源识别复合物（ORC）占据。有人提议Dbf4p在将G1 / S激酶Cdc7p靶向G1晚期的起始复合体中发挥作用。我们报告Dbf4p可能也招募Cdc5p起源复合体。 Cdc5p是完成有丝分裂所需的Polo激酶家族的成员。 Cdc5p和Cdc7p各自与Dbf4p的不同域相互作用。 cdc5-1突变体具有质粒维持缺陷，可以通过添加多个起点来抑制。 cdc5-1 orc2-1双重突变体具有合成致命性。发现Cdc5p的水平受细胞周期调节，并在G2 / M中达到峰值。这些结果表明，Cdc5p和起源复合物上的Polo样激酶可能发挥了作用。

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