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首页> 外文期刊>Molecular and Cellular Biology >Interaction of yeast repressor-activator protein Ume6p with glycogen synthase kinase 3 homolog Rim11p.
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Interaction of yeast repressor-activator protein Ume6p with glycogen synthase kinase 3 homolog Rim11p.

机译：酵母阻遏物激活蛋白Ume6p与糖原合酶激酶3同源Rim11p的相互作用。

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摘要

Meiosis and expression of early meiotic genes in the budding yeast Saccharomyces cerevisiae depend upon Rim11p, Ume6p, and Ime1p. Rim11p (also called Mds1p and ScGSK3) is a protein kinase related to glycogen synthase kinase 3 (GSK3); Ume6p is an architectural transcription factor; and Imelp is a Ume6p-binding protein that provides a transcriptional activation domain. Rim11p is required for Ime1p-Ume6p interaction, and prior studies have shown that Rim11p binds to and phosphorylates Ime1p. We show here that Rim11p binds to and phosphorylates Ume6p, as well. Amino acid substitutions in Ume6p that alter a consensus GSK3 site reduce or abolish Rim11p-Ume6p interaction and Rim11p-dependent phosphorylation, and they cause defects in interaction between Ume6p and Ime1p and in meiotic gene expression. Therefore, interaction between Rim11p and Ume6p, resulting in phosphorylation of Ume6p, is required for Ime1p-Ume6p complex formation. Rim11p, like metazoan GSK3beta, phosphorylates both interacting subunits of a target protein complex.
机译：萌芽酵母酿酒酵母中的减数分裂和早期减数分裂基因的表达取决于Rim11p，Ume6p和Ime1p。 Rim11p（也称为Mds1p和ScGSK3）是与糖原合酶激酶3（GSK3）相关的蛋白激酶。 Ume6p是一种建筑转录因子; Imelp是提供转录激活结构域的Ume6p结合蛋白。 Rim11p是Ime1p-Ume6p相互作用所必需的，以前的研究表明Rim11p结合并磷酸化Ime1p。我们在这里显示，Rim11p也与Ume6p结合并使其磷酸化。 Ume6p中的氨基酸替换可改变共有的GSK3位点，从而减少或消除Rim11p-Ume6p相互作用和Rim11p依赖的磷酸化作用，它们在Ume6p和Ime1p之间的相互作用以及减数分裂基因表达中引起缺陷。因此，Ime1p-Ume6p复合物的形成需要Rim11p和Ume6p之间的相互作用，导致Ume6p磷酸化。像后生动物GSK3beta一样，Rim11p使靶蛋白复合物的两个相互作用的亚基磷酸化。

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